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AuthorBen Abdallah, Manel
AuthorKarray, Fatma
AuthorSayadi, Sami
Available date2020-09-22T09:24:48Z
Publication Date2020
Publication NameBiomolecules
CitationBen Abdallah, M.; Karray, F.; Sayadi, S. Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources. Biomolecules 2020, 10, 109.
URIhttp://dx.doi.org/10.3390/biom10010109
URIhttp://hdl.handle.net/10576/16223
AbstractThe large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme environment, the salt lake Chott El Jerid for the accumulation of these inclusions. Among them, two archaeal strains showed positive results with phenotypic and genotypic methods. Phylogenetic analysis, based on the 16S rRNA gene, indicated that polyhydroxyalkanoate (PHA)-producing archaeal isolates CEJGTEA101 and CEJEA36 were related to Natrinema altunense and Haloterrigena jeotgali, respectively. Gas chromatography and UV-visible spectrophotometric analyses revealed that the PHA were identified as polyhydroxybutyrate and polyhydroxyvalerate, respectively. According to gas chromatography analysis, the strain CEJGTEA101 produced maximum yield of 7 wt % at 37 °C; pH 6.5; 20% NaCl and the strain CEJEA36 produced 3.6 wt % at 37 °C; pH 7; 25% NaCl in a medium supplemented with 2% glucose. Under nutritionally optimal cultivation conditions, polymers were extracted from these strains and were determined by gravimetric analysis yielding PHA production of 35% and 25% of cell dry weight. In conclusion, optimization of PHA production from inexpensive industrial wastes and carbon sources has considerable interest for reducing costs and obtaining high yield
Languageen
PublisherMDPI AG
Subjectpolyhydroxyalkanoate
biodegradable polymer
hypersaline lake
PHA-producing archaea
carbon sources
TitleProduction of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources
TypeArticle
Pagination14-Feb
Issue Number1
Volume Number10
dc.accessType Open Access


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