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المؤلفLópez-Ozuna, Vanessa M
المؤلفGupta, Ishita
المؤلفKiow, Ryan Liu Chen
المؤلفMatanes, Emad
المؤلفKheraldine, Hadeel
المؤلفYasmeen, Amber
المؤلفKhalil, Ashraf
المؤلفVranic, Semir
المؤلفAl Moustafa, Ala-Eddin
المؤلفFarsi, Halema F Al
تاريخ الإتاحة2020-09-27T05:02:38Z
تاريخ النشر2020-09-18
اسم المنشورToxics
المعرّفhttp://dx.doi.org/10.3390/toxics8030073
الاقتباسLópez-Ozuna, V.M.; Gupta, I.; Kiow, R.L.C.; Matanes, E.; Kheraldine, H.; Yasmeen, A.; Khalil, A.; Vranic, S.; Al Moustafa, A.-E.; Farsi, H.F.A. Water-Pipe Smoking Exposure Deregulates a Set of Genes Associated with Human Head and Neck Cancer Development and Prognosis. Toxics 2020, 8, 73.
معرّف المصادر الموحدhttp://hdl.handle.net/10576/16308
الملخصWater-pipe smoking (WPS) is becoming the most popular form of tobacco use among the youth, especially in the Middle East, replacing cigarettes rapidly and becoming a major risk of tobacco addiction worldwide. Smoke from WPS contains similar toxins as those present in cigarette smoke and is linked directly with different types of cancers including lung and head and neck (HN) carcinomas. However, the underlying molecular pathways and/or target genes responsible for the carcinogenic process are still unknown. In this study, human normal oral epithelial (HNOE) cells, NanoString PanCancer Pathways panel of 770 gene transcripts and quantitative real-time polymerase chain reaction (qRT-PCR) analysis were applied to discover differentially expressed genes (DEG) modulated by WPS. In silico analysis was performed to analyze the impact of these genes in HN cancer patient's biology and outcome. We found that WPS can induce the epithelial-mesenchymal transition (EMT: hallmark of cancer progression) of HNOE cells. More significantly, our analysis of NanoString revealed 23 genes deregulated under the effect of WPS, responsible for the modulation of cell cycle, proliferation, migration/invasion, apoptosis, signal transduction, and inflammatory response. Further analysis was performed using qRT-PCR of HNOE WPS-exposed and unexposed cells supported the reliability of our NanoString data. Moreover, we demonstrate those DEG to be upregulated in cancer compared with normal tissue. Using the Kaplan-Meier analysis, we observed a significant association between WPS-deregulated genes and relapse-free survival/overall survival in HN cancer patients. Our findings imply that WPS can modulate EMT as well as a set of genes that are directly involved in human HN carcinogenesis, thereby affecting HN cancer patients' survival.
راعي المشروعQatar University, grant numbers: QUCP-CMED-2019-1, QUCG-CMED-20/21-2 & QUHI-CMED-19/20-1.
اللغةen
الناشرMDPI
الموضوعgene dysregulation
head and neck cancers
oral epithelial cells
smoke
water pipe
العنوانWater-Pipe Smoking Exposure Deregulates a Set of Genes Associated with Human Head and Neck Cancer Development and Prognosis.
النوعArticle
رقم العدد3
رقم المجلد8
ESSN2305-6304
dc.accessType Open Access


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