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AuthorAlHiraky, H
AuthorMoulana, N
AuthorRiahi, M
AuthorAlsowaidi, K
AuthorAlbukhati, K
AuthorZughaie, SM
AuthorEltai, NO
Available date2021-08-19T06:59:41Z
Publication Date2021-07-22
Publication NameJournal of Bacteriology and Mycology
CitationShams N, AlHiraky H, Moulana N, Riahi M, Alsowaidi K, Albukhati K, et al. Comparing Quantitative and Qualitative Methods for Detecting the In Vitro Activity of Colistin against Different Gram-Negative Bacilli. J Bacteriol Mycol. 2021; 8(5): 1181
ISSN2471-0172
Identifier1181
URIhttps://austinpublishinggroup.com/bacteriology/
URIhttp://hdl.handle.net/10576/22046
AbstractThe surge in the prevalence of Multidrug-Resistant (MDR) Gram-negative bacterial infections with limited treatment led to colistin reusing to treat MDR infections. This study aimed to determine economical, simple, and reliable colistin susceptibility testing methods as an alternative to the microdilution technique. We compared seven colistin susceptibility testing methods, including quantitative and qualitative, namely: Disk diffusion, E-test, ComASPTM SensiTest Colistin, Colistin broth disk elution, and colistin agar test CHROMagarTM COL-APSE, and BD Phoenix ID/AST automated identification and susceptibility testing system to the gold standard Broth Microdilution (BMD). Whole-genome sequencing was performed on all isolates to determine if the genetic resistant factors affect the phenotypic profile of the colistin resistance. Our results revealed that disk diffusion is still an ineffective method for measuring colistin susceptibility in Gram-negative Bacilli with the highest major error (31.75%), the lowest Kappa 0 (0%), and categorical agreement (68.25%) values. Phoenix, and CompASPTM SensiTest colistin methods have remained superior in reproducibility, sturdiness, and simplicity of use, similar to the currently recommended broth microdilution procedure; with high sensitivity of 95.56%, and 97.73%, specificity of 95.24, and 100%, and Kappa values of 0.89 and 0.95, respectively. This study revealed that Phoenix, and ComASPTM SensiTest colistin methods
Sponsor(UREP26-024-1-002)from the Qatar National Research Fund (QNRF
Languageen
PublisherAustin Publishing Group
SubjectColistin
SubjectGram-negative bacilli
SubjectResistance
SubjectMicrodilution
SubjectMIC
TitleComparing Quantitative and Qualitative Methods for Detecting the In Vitro Activity of Colistin against Different Gram-Negative Bacilli
TypeArticle
Issue Number5
Volume Number8


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