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AuthorAl Mana, Hassan
AuthorSundararaju, Sathyavathi
AuthorTsui, Clement K. M.
AuthorPerez-Lopez, Andres
AuthorYassine, Hadi
AuthorAl Thani, Asmaa
AuthorAl-Ansari, Khalid
AuthorEltai, Nahla O
Available date2021-08-19T07:21:52Z
Publication Date2021-08-12
Publication NameAntibiotics
Identifierhttp://dx.doi.org/10.3390/antibiotics10080972
CitationAl Mana, H.; Sundararaju, S.; Tsui, C.K.M.; Perez-Lopez, A.; Yassine, H.; Al Thani, A.; Al-Ansari, K.; Eltai, N.O. Whole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients. Antibiotics 2021, 10, 972. https://doi.org/10.3390/ antibiotics10080972
Identifier972
URIhttp://hdl.handle.net/10576/22073
AbstractAntibiotic resistance is a growing public health problem globally, incurring health and cost burdens. The occurrence of antibiotic-resistant bacterial infections has increased significantly over the years. Gram-negative bacteria display the broadest resistance range, with bacterial species expressing extended-spectrum -lactamases (ESBLs), AmpC, and carbapenemases. All carbapenem-resistant Enterobacteriaceae (CRE) isolates from pediatric urinary tract infections (UTIs) between October 2015 and November 2019 (n = 30). All isolates underwent antimicrobial resistance phenotypic testing using the Phoenix NMIC/ID-5 panel, and carbapenemase production was confirmed using the NG-Test CARBA 5 assay. Whole-genome sequencing was performed on the CREs. The sequence type was identified using the Achtman multi-locus sequence typing scheme, and antimicrobial resistance markers were identified using ResFinder and the CARD database. The most common pathogens causing CRE UTIs were E. coli (63.3%) and K. pneumoniae (30%). The most common carbapenemases produced were OXA-48-like enzymes (46.6%) and NDM enzymes (40%). Additionally, one E. coliharbored IMP-26, and two K. pneumoniae possessed mutations in ompK37 and/or ompK36. Lastly,one E. coli had a mutation in the marA porin and efflux pump regulator. The findings highlight the difference in CRE epidemiology in the pediatric population compared to Qatar’s adult population,where NDM carbapenemases are more common.
SponsorQatar University, grant number “QUUG-BRC-2017
Languageen
PublisherMDPI
Subjectcarbapenem-resistance
Enterobacteriaceae
Qatar
CRE
OXA-48
TitleWhole-Genome Sequencing for Molecular Characterization of Carbapenem-Resistant Enterobacteriaceae Causing Lower Urinary Tract Infection among Pediatric Patients
TypeArticle
Issue Number8
Volume Number10
dc.accessType Open Access


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