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AuthorBertok, Tomas
AuthorDosekova, Erika
AuthorBelicky, Stefan
AuthorHolazova, Alena
AuthorLorencova, Lenka
AuthorMislovicova, Danica
AuthorPaprckova, Darina
AuthorVikartovska, Alica
AuthorPlicka, Robert
AuthorKrejci, Jan
AuthorIlcikova, Marketa
AuthorKasak, Peter
AuthorTkac, Jan
Available date2021-09-01T10:02:43Z
Publication Date2016
Publication NameLangmuir
ResourceScopus
URIhttp://dx.doi.org/10.1021/acs.langmuir.6b01456
URIhttp://hdl.handle.net/10576/22368
AbstractAn impedimetric lectin biosensor for the detection of changes in the glycan structure of antibodies isolated from human serum is here correlated with the progression of rheumatoid arthritis (RA). The biosensor was built up from a mixed self-assembled monolayer (SAM) on gold consisting of two different thiolated zwitterionic derivatives, carboxybetaine and sulfobetaine, to resist nonspecific interactions. The carboxyl-terminated one was applied also for the covalent immobilization of lectin Ricinus communis agglutinin I (RCA-I). The process of building a bioreceptive layer was optimized and characterized using a diverse range of techniques. Impedimetric assays were integrated on a chip consisting of eight gold working electrodes, which is an important step toward the achievement of a moderate level of multiplexing for the analysis of human serum samples. At the end, the results obtained by the impedimetric analysis of immunoglobulins G (IgGs) isolated from serum samples were compared with those of two other standard bioanalytical methods employing lectins, that is, lectin microarrays (MAs) and enzyme-linked lectin binding assays (ELLBAs). The impedimetric results agreed very well with the DAS28 index (RA disease activity score 28), suggesting that impedimetric assays could be used for the development of a new diagnostic procedure sensitive to glycosylation changes in human IgGs and thus RA progression. 2016 American Chemical Society.
Languageen
PublisherAmerican Chemical Society
SubjectBiosensors
Body fluids
Chemical detection
Diagnosis
Diseases
Gold
Monolayers
Proteins
Bioanalytical methods
Covalent immobilization
Detection of changes
Diagnostic procedure
Lectin microarrays
Mixed self assembled monolayers
Non-specific interactions
Rheumatoid arthritis
Self assembled monolayers
immunoglobulin G
plant lectin
polysaccharide
Ricinus communis agglutinin-1
blood
chemistry
devices
electrode
genetic procedures
glycosylation
human
immunoassay
procedures
protein microarray
rheumatoid arthritis
Arthritis, Rheumatoid
Biosensing Techniques
Electrodes
Glycosylation
Humans
Immunoassay
Immunoglobulin G
Plant Lectins
Polysaccharides
Protein Array Analysis
TitleMixed Zwitterion-Based Self-Assembled Monolayer Interface for Impedimetric Glycomic Analyses of Human IgG Samples in an Array Format
TypeArticle
Pagination7070-7078
Issue Number28
Volume Number32
dc.accessType Abstract Only


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