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AuthorKasák, Peter
AuthorMosnáček, Jaroslav
AuthorDanko, Martin
AuthorKrupa, Igor
AuthorHloušková, Gabriela
AuthorChorvát, Dušan
AuthorKoukaki, Marina
AuthorKaramanou, Spyridoula
AuthorEconomou, Anastassios
AuthorLacík, Igor
Available date2021-09-08T06:49:45Z
Publication Date2016
Publication NameRSC Advances
ResourceScopus
ISSN20462069
URIhttp://dx.doi.org/10.1039/c6ra14423c
URIhttp://hdl.handle.net/10576/22915
AbstractA hydrogel based on sulfobetaine methacrylate monomer N-(methacryloyloxyethyl)-N,N-dimethyl-N-(3-sulfopropyl)ammonium betaine and N,N-bis(methacryloyloxyethyl)-N-methyl-N-(3-sulfopropyl)ammonium betaine used as a crosslinker was investigated as a potential material for biosensor applications. The glucose diffusion coefficient of 1.2 × 10−10 m2 s−1 was determined from the glucose release experiment. Inverse size-exclusion chromatography was performed to determine the molecular weight cut-off of the hydrogel to be 8 kDa with respect to pullulans that corresponds to a viscosity radius of 2.1 nm. The narrow pore-size distribution suggests that using the sulfobetaine crosslinker suppresses the composition drift and results in a homogeneous hydrogel network. Furthermore, a glucose biosensor construct comprising the periplasmic glucose-binding protein of Escherichia coli fused to cyan and yellow fluorescent proteins was effectively entrapped in the hydrogel exhibiting no leakage for at least 7 days. The glucose-binding protein showed stability of its secondary structure and sensitivity to glucose as assessed by circular dichroism and Förster (fluorescence) resonance energy transfer measurements under physiological conditions and a physiological range of glucose concentration, respectively.
Languageen
PublisherRoyal Society of Chemistry
SubjectBetaines
Binding energy
Bins
Biosensors
Dichroism
Energy transfer
Escherichia coli
Fluorescence
Glucose
Glucose sensors
Physiology
Pore size
Proteins
Size exclusion chromatography
Biosensor applications
Glucose concentration
Inverse size exclusion chromatography
Molecular weight cutoff
Narrow pore size distributions
Physiological condition
Resonance energy transfer
Yellow fluorescent proteins
Hydrogels
TitleA polysulfobetaine hydrogel for immobilization of a glucose-binding protein
TypeArticle
Pagination83890-83900
Issue Number87
Volume Number6
dc.accessType Abstract Only


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