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AuthorShurrab, Farah M
AuthorAl-Sadeq, Duaa W
AuthorAmanullah, Fatima Humaira
AuthorAl-Absi, Enas S
AuthorQotba, Hamda
AuthorYassine, Hadi M
AuthorAbu-Raddad, Laith J
AuthorNasrallah, Gheyath K
Available date2022-03-13T04:59:16Z
Publication Date2022-02-08
Publication NameIntervirology
Identifierhttp://dx.doi.org/10.1159/000522479
CitationShurrab F, M, Al-Sadeq D, W, Amanullah F, H, Al-Absi E, S, Qotba H, Yassine H, M, Abu-Raddad L, J, Nasrallah G, K: Low Risk of Serological Cross-Reactivity between the Dengue Virus and SARS-CoV-2-IgG Antibodies Using Advanced Detection Assays. Intervirology 2022. doi: 10.1159/000522479
ISSN0300-5526
URIhttp://hdl.handle.net/10576/27985
AbstractSeveral studies have reported serological cross-reactivity of the immune responses between SARS-CoV-2 and DENV. Most of the available studies are based on the point of care (POC) rapid testing kits. However, some rapid test kits have low specificity and can generate false positives. Hence, we aimed to investigate the potential serological cross-reactivity between SARS-CoV-2 and DENV IgG antibodies using advanced assays including chemiluminescence immunoassay (CLIA) and ELISA test. A total of 90 DENV-IgG-ELISA positive and 90 negative pre-pandemic sera were tested for anti-SARS-CoV-2-IgG using the automated CL-900i CLIA assay. Furthermore, a total of 91 SARS-CoV-2-IgG-CLIA positive and 91 negative post-pandemic sera were tested for anti-DENV-IgG using the Novalisa ELISA assay. The DENV-IgG positive sera resulted in five positives and 85 negatives for SARS-CoV-2-IgG. Similarly, the DENV-IgG negative sera also resulted in five positives and 85 negatives for SARS-CoV-2-IgG. No statistically significant difference in specificity between the DENV-IgG positive and DENV-IgG negative sera was found (p-value=1.00). The SARS-CoV-2-IgG positive sera displayed 43 positives, 47 negatives, and one equivocal for DENV-IgG. Whereas the SARS-CoV-2-IgG negative sera resulted in 50 positives, 40 negatives, and one equivocal for DENV-IgG. No statistically significant difference in the proportion that is DENV-IgG positive between the SARS-CoV-2-IgG positive and SARS-CoV-2-IgG negative sera (p-value=0.58). In conclusion, there is a low risk of serological cross-reactivity between the DENV, and SARS-CoV-2 IgG antibodies when using advanced detection assays.  .
SponsorL.J.A. acknowledges the support of the Biomedical Research Program and the Biostatistics, Epidemiology, and Biomathematics Research Core, both at Weill Cornell Medicine-Qatar. This work was made possible by Grant Nos. RRC-2-032 and UREP19-013-3-001 from the Qatar National Research Fund (a member of Qatar Foundation). The statements made herein are solely the responsibility of the authors. In addition, G.K.N. would also like to acknowledge funds from Qatar University’s internal Grant QUERG-CMED-2020-2.
Languageen
PublisherKarger
Subjectcross-reactivity
dengue
Severe acute respiratory syndrome coronavirus 2
Chemiluminescence immunoassay
Enzyme-linked immunosorbent assay
TitleLow risk of serological cross-reactivity between the dengue virus and SARS-CoV-2 IgG antibodies using advanced detection assays.
TypeReport
ESSN1423-0100
dc.accessType Open Access


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