عرض بسيط للتسجيلة

المؤلفMelavanki, Raveendra
المؤلفSharma, Kalpana
المؤلفYallur, Basappa Chanabasapa
المؤلفKusanur, Raviraj
المؤلفSadasivuni, Kishor Kumar
المؤلفSingh, Diksha
المؤلفMane, Smita
المؤلفKatagi, Kariyappa
المؤلفPattar, Shridhar V.
تاريخ الإتاحة2022-03-23T06:35:48Z
تاريخ النشر2021
اسم المنشورLuminescence
المصدرScopus
المعرّفhttp://dx.doi.org/10.1002/bio.3931
الاقتباسMelavanki, R, Sharma, K, Yallur, BC, et al. Understanding the binding interaction between phenyl boronic acid P1 and sugars: determination of association and dissociation constants using S-V plots, steady-state spectroscopic methods and molecular docking. Luminescence. 2021; 36: 163- 168. https://doi.org/10.1002/bio.3931
معرّف المصادر الموحدhttp://hdl.handle.net/10576/28590
الملخصContinuous monitoring of glucose and sugar sensing plays a vital role in diabetes control. The drawbacks of the present enzyme-based sugar sensors have encouraged the investigation into alternate approaches to design new sensors. The popularity of fluorescence sensors is due to their ability to bind reversibly to compounds containing diol. In this study we investigated the binding ability of phenyl boronic acid P1 for monosaccharides and disaccharides (sugars) in aqueous medium at physiological pH 7.4 using steady-state fluorescence and absorbance. P1 fluorescence was quenched due to formation of esters with sugars. Absorbance and fluorescence measurements led to results that indicated that the sugars studied could be ordered in terms of their affinity to P1, as stated: sucrose > lactose > galactose > xylose > ribose > arabinose. In each case, the slope of modified Stern?Volmer plots was nearly 1, indicating the presence of only a single binding site in boronic acids for sugars. Docking studies were carried out using Schrodinger Maestro v.11.2 software. The binding affinity of phenyl boronic acid P1 with periplasmic protein (PDB ID 2IPM and 2IPL) was estimated using GlideScore.
اللغةen
الناشرJohn Wiley and Sons Ltd
الموضوعbinding constant
dissociation constant
fluorescence intensity
glucose level
phenyl boronic acid
sugars
S?V plot
العنوانUnderstanding the binding interaction between phenyl boronic acid P1 and sugars: determination of association and dissociation constants using S?V plots, steady-state spectroscopic methods and molecular docking
النوعArticle
الصفحات163-168
رقم العدد1
رقم المجلد36
dc.accessType Abstract Only


الملفات في هذه التسجيلة

الملفاتالحجمالصيغةالعرض

لا توجد ملفات لها صلة بهذه التسجيلة.

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة