Show simple item record

AuthorMelavanki, Raveendra
AuthorSharma, Kalpana
AuthorYallur, Basappa Chanabasapa
AuthorKusanur, Raviraj
AuthorSadasivuni, Kishor Kumar
AuthorSingh, Diksha
AuthorMane, Smita
AuthorKatagi, Kariyappa
AuthorPattar, Shridhar V.
Available date2022-03-23T06:35:48Z
Publication Date2021
Publication NameLuminescence
ResourceScopus
Identifierhttp://dx.doi.org/10.1002/bio.3931
CitationMelavanki, R, Sharma, K, Yallur, BC, et al. Understanding the binding interaction between phenyl boronic acid P1 and sugars: determination of association and dissociation constants using S-V plots, steady-state spectroscopic methods and molecular docking. Luminescence. 2021; 36: 163- 168. https://doi.org/10.1002/bio.3931
URIhttp://hdl.handle.net/10576/28590
AbstractContinuous monitoring of glucose and sugar sensing plays a vital role in diabetes control. The drawbacks of the present enzyme-based sugar sensors have encouraged the investigation into alternate approaches to design new sensors. The popularity of fluorescence sensors is due to their ability to bind reversibly to compounds containing diol. In this study we investigated the binding ability of phenyl boronic acid P1 for monosaccharides and disaccharides (sugars) in aqueous medium at physiological pH 7.4 using steady-state fluorescence and absorbance. P1 fluorescence was quenched due to formation of esters with sugars. Absorbance and fluorescence measurements led to results that indicated that the sugars studied could be ordered in terms of their affinity to P1, as stated: sucrose > lactose > galactose > xylose > ribose > arabinose. In each case, the slope of modified Stern?Volmer plots was nearly 1, indicating the presence of only a single binding site in boronic acids for sugars. Docking studies were carried out using Schrodinger Maestro v.11.2 software. The binding affinity of phenyl boronic acid P1 with periplasmic protein (PDB ID 2IPM and 2IPL) was estimated using GlideScore.
Languageen
PublisherJohn Wiley and Sons Ltd
Subjectbinding constant
dissociation constant
fluorescence intensity
glucose level
phenyl boronic acid
sugars
S?V plot
TitleUnderstanding the binding interaction between phenyl boronic acid P1 and sugars: determination of association and dissociation constants using S?V plots, steady-state spectroscopic methods and molecular docking
TypeArticle
Pagination163-168
Issue Number1
Volume Number36
dc.accessType Abstract Only


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record