Staphylococcus aureus histone deacteylase-like enzyme is a potential target for adjuvant antibiotic discovery
المؤلف | Chowdhury, Shazeda H. |
المؤلف | Zughaier, Susu M. |
تاريخ الإتاحة | 2022-03-24T05:33:53Z |
تاريخ النشر | 2022 |
اسم المنشور | Journal of Emergency Medicine, Trauma and Acute Care |
المصدر | qscience |
الاقتباس | Chowdhury SH, Zughaier SM. Staphylococcus aureus histone deacteylase-like enzyme is a potential target for adjuvant antibiotic discovery, Journal of Emergency Medicine, Trauma & Acute Care 2022:37 http://dx.doi.org/10.5339/jemtac.2022.qhc.37 |
الرقم المعياري الدولي للكتاب | 1999-7086 |
الرقم المعياري الدولي للكتاب | 1999-7094 |
الملخص | Background: The rise in antibiotic resistance requires prompt action to reduce the burden of untreatable bacterial diseases1. Staphylococcus aureus is a human commensal and opportunistic pathogen that causes a broad range of diseases, from mild skin infections to infective endocarditis. The World Health Organization has placed S. aureus on the high-priority pathogen list due to its multidrug-resisting nature2. The study aims to identify molecular targets for antibiotic adjuvants to restore antibiotic activity. Methods: Extensive blast search and computational analysis were employed to search published S. aureus genomes. The effect of suggested adjuvants was tested on sensitive and resistant S. aureus strains in-vitro. Bacteria were incubated in the presence of either an HDAC inhibitor (TSA) or an antibiotic (Cefixime), or a combination of both. Results: A gene that encodes a histone deacetylase-like enzyme (SA-HDAC) and shares high 3D-homology to human HDAC2 and HDAC8 was identified3. Using computational modeling, it was found that the SA-HDAC protein has an active catalytic pocket containing the highly conserved zinc-binding constellation, suggesting an HDAC-like activity. I-TASSER analysis revealed that HDAC inhibitors such as TSA, CRI, LLX, NHB, and B3N can bind to the catalytic core. From the growth curves generated using the in-vitro study, it was observed that while Cefixime alone had no effect, TSA had an inhibitory effect, and the combination showed an additive effect on both strains. Further, the effect was more evident in the sensitive strain as compared to MRSA. An extensive bioinformatics blast search showed that this gene is absent in most gut microbiota species but found in many pathogens that carry and spread multidrug resistance in healthcare settings as well as in community-acquired infections. Conclusion: SA-HDAC enzyme, which is absent in most gut microbiota, is a highly druggable target that can be utilized for novel adjuvant antibiotic discovery. |
اللغة | en |
الناشر | Hamad bin Khalifa University Press (HBKU Press) |
الموضوع | Adjuvant Antimicrobial resistance Histone deacetylase Staphylococcus aureus Trichostatin A |
النوع | Article |
رقم العدد | 1 |
رقم المجلد | 2022 |
الملفات في هذه التسجيلة
هذه التسجيلة تظهر في المجموعات التالية
-
أبحاث الطب [1418 items ]