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AuthorChowdhury, Shazeda H.
AuthorZughaier, Susu M.
Available date2022-03-24T05:33:53Z
Publication Date2022
Publication NameJournal of Emergency Medicine, Trauma and Acute Care
Resourceqscience
CitationChowdhury SH, Zughaier SM. Staphylococcus aureus histone deacteylase-like enzyme is a potential target for adjuvant antibiotic discovery, Journal of Emergency Medicine, Trauma & Acute Care 2022:37 http://dx.doi.org/10.5339/jemtac.2022.qhc.37
ISSN1999-7086
ISSN1999-7094
URIhttps://doi.org/10.5339/jemtac.2022.qhc.37
URIhttp://hdl.handle.net/10576/28920
AbstractBackground: The rise in antibiotic resistance requires prompt action to reduce the burden of untreatable bacterial diseases1. Staphylococcus aureus is a human commensal and opportunistic pathogen that causes a broad range of diseases, from mild skin infections to infective endocarditis. The World Health Organization has placed S. aureus on the high-priority pathogen list due to its multidrug-resisting nature2. The study aims to identify molecular targets for antibiotic adjuvants to restore antibiotic activity. Methods: Extensive blast search and computational analysis were employed to search published S. aureus genomes. The effect of suggested adjuvants was tested on sensitive and resistant S. aureus strains in-vitro. Bacteria were incubated in the presence of either an HDAC inhibitor (TSA) or an antibiotic (Cefixime), or a combination of both. Results: A gene that encodes a histone deacetylase-like enzyme (SA-HDAC) and shares high 3D-homology to human HDAC2 and HDAC8 was identified3. Using computational modeling, it was found that the SA-HDAC protein has an active catalytic pocket containing the highly conserved zinc-binding constellation, suggesting an HDAC-like activity. I-TASSER analysis revealed that HDAC inhibitors such as TSA, CRI, LLX, NHB, and B3N can bind to the catalytic core. From the growth curves generated using the in-vitro study, it was observed that while Cefixime alone had no effect, TSA had an inhibitory effect, and the combination showed an additive effect on both strains. Further, the effect was more evident in the sensitive strain as compared to MRSA. An extensive bioinformatics blast search showed that this gene is absent in most gut microbiota species but found in many pathogens that carry and spread multidrug resistance in healthcare settings as well as in community-acquired infections. Conclusion: SA-HDAC enzyme, which is absent in most gut microbiota, is a highly druggable target that can be utilized for novel adjuvant antibiotic discovery.
Languageen
PublisherHamad bin Khalifa University Press (HBKU Press)
SubjectAdjuvant
Antimicrobial resistance
Histone deacetylase
Staphylococcus aureus
Trichostatin A
TitleStaphylococcus aureus histone deacteylase-like enzyme is a potential target for adjuvant antibiotic discovery
TypeArticle
Issue Number1
Volume Number2022
dc.accessType Open Access


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