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AuthorRashidi, Moloud
AuthorTavalaee, Marziyeh
AuthorAbbasi, Homayon
AuthorNomikos, Michail
AuthorNasr-Esfahani, Mohammad Hossein
Available date2022-04-24T10:54:14Z
Publication Date2021-09-01
Publication NameCell Journal
Identifierhttp://dx.doi.org/10.22074/cellj.2021.7265
CitationRashidi Moloud, Tavalaee Marziyeh, Abbasi Homayon, Nomikos Michail, Nasr-Esfahani Mohammad Hossein. Increased de novo DNA Methylation Enzymes in Sperm of Individuals with Varicocele. Cell J. 2021; 23(4): 389-396.
ISSN22285806
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85115061156&origin=inward
URIhttp://hdl.handle.net/10576/30248
AbstractObjective: Chronic genital heat-stress associated with varicocele leads to DNA hypo-methylation of spermatozoa. The objective of this study was comparing level of DNA methyl-transferases (DNMTs) in sperm of men suffering varicocele with fertile individuals. Materials and Methods: In this case-control study, semen samples were obtained from 35 infertile men with varicocele (grade II or III) and 26 fertile men. Sperm parameters were assessed according to World Health Organization (WHO) protocol. DNMTs enzymes level were assessed by flow cytometer and fluorescence microscope. mRNAs expression of these DNMTs were also assessed by real-time reverse transcription polymerase chain reaction (RT-PCR). Results: DNMT1 and DNMT3A proteins were mainly localized in equatorial and mid-piece regions of sperm head, respectively, while DNMT3B protein appeared to be localized mainly in equatorial and anterior regions of sperm head. In contrast to DNMT1, expression and percentage of DNMT3A and DNMT3B at RNA and protein levels were significantly higher in the varicocele group compared to the fertile group (P<0.05). In addition, significant correlations were found between sperm concentration and motility as well as DNMT1 and DNMT3B proteins levels in the infertile individuals with varicocele (P<0.05). Additionally, significant correlations were observed between abnormal sperm morphology with DNMTs proteins in the infertile individuals with varicocele. Conclusion: Unlike DNMT1, which is involved in maintenance of DNA methylation at both RNA and protein levels, expression of de novo methylation enzymes (DNMT3A and DNMT3B) at both levels were increased in the varicocele group compared to the fertile group. Based on literature, this increase might be due to the dual roles played by DNMT3A and DNMT3B, as methyl-transferases in normal condition as well as dehydroxymethylases in stress condition, like varicocele. Although, this hypothesis needs further validation.
Languageen
Publisherpmc
SubjectDNA methylation
DNMT1
DNMT3A
DNMT3B
Varicocele
TitleIncreased de novo DNA methylation enzymes in sperm of individuals with varicocele
TypeArticle
Pagination389-396
Issue Number4
Volume Number23
dc.accessType Open Access


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