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المؤلفAbdo, Ghada G.
المؤلفGupta, Ishita
المؤلفKheraldine, Hadeel
المؤلفRizeq, Balsam
المؤلفZagho, Moustafa M.
المؤلفKhalil, Ashraf
المؤلفElzatahry, Ahmed
المؤلفAl Moustafa, Ala-Eddin
تاريخ الإتاحة2022-05-22T09:12:23Z
تاريخ النشر2021
اسم المنشورMaterials Science and Engineering C
المصدرScopus
المعرّفhttp://dx.doi.org/10.1016/j.msec.2021.111910
معرّف المصادر الموحدhttp://hdl.handle.net/10576/31352
الملخصCarbon nanofibers (CNFs) have been implicated in biomedical applications, yet, they are still considered as a potential hazard. Conversely, mesoporous silica is a biocompatible compound that has been used in various biomedical applications. In this regard, we recently reported that CNFs induce significant toxicity on the early stage of embryogenesis in addition to the inhibition of its angiogenesis. Thus, we herein use mesoporous silica coating of CNFs (MCNFs) in order to explore their outcome on normal development and angiogenesis using avian embryos at 3 days and its chorioallantoic membrane (CAM) at 6 days of incubation. Our data show that mesoporous silica coating of CNFs significantly reduces embryotoxicity provoked by CNFs. However, MCNFs exhibit slight increase in angiogenesis inhibition in comparison with CNFs. Further investigation revealed that MCNFs slightly deregulate the expression patterns of key controller genes involved in cell proliferation, survival, angiogenesis, and apoptosis as compared to CNFs. We confirmed these data using avian primary normal embryonic fibroblast cells established in our lab. Regarding the molecular pathways, we found that MCNFs downregulate the expression of ERK1/ERK2, p-ERK1/ERK2 and JNK1/JNK2/JNK3, thus indicating a protective role of MCNFs via ERK and JNK pathways. Our data suggest that coating CNFs with a layer of mesoporous silica can overcome their toxicity making them suitable for use in biomedical applications. Nevertheless, further investigations are required to evaluate the effects of MCNFs and their mechanisms using different in vitro and in vivo models. 2021 Elsevier B.V.
اللغةen
الناشرElsevier Ltd
الموضوعBiocompatibility
Biohazards
Carbon nanofibers
Cell culture
Cell death
Cell proliferation
Coatings
Medical applications
Silica
Toxicity
Biomedical applications
Chorio-allantoic membranes
Embryonic fibroblasts
Embryotoxicity
Expression patterns
Mesoporous Silica
Molecular pathways
Potential hazards
Mesoporous materials
carbon
nanofiber
silicon dioxide
animal
chorioallantois
MAPK signaling
Animals
Carbon
Chorioallantoic Membrane
MAP Kinase Signaling System
Nanofibers
Silicon Dioxide
العنوانMesoporous silica coated carbon nanofibers reduce embryotoxicity via ERK and JNK pathways
النوعArticle
رقم المجلد122
dc.accessType Abstract Only


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