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    SARS-CoV-2 RNA Recovery from Air Sampled on Quartz Fiber Filters: A Matter of Sample Preservation?

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    SARS-CoV-2 RNA Recovery from Air Sampled (1.877Mb)
    Date
    2022-02-01
    Author
    Licen, Sabina
    Zupin, Luisa
    Martello, Lorenzo
    Torboli, Valentina
    Semeraro, Sabrina
    Gardossi, Anna Lilian
    Greco, Enrico
    Fontana, Francesco
    Crovella, Sergio
    Ruscio, Maurizio
    Palmisani, Jolanda
    Di Gilio, Alessia
    Piscitelli, Prisco
    Pallavicini, Alberto
    Barbieri, Pierluigi
    ...show more authors ...show less authors
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    Abstract
    The airborne route of transmission of SARS-CoV-2 was confirmed by the World Health Organization in April 2021. There is an urge to establish standardized protocols for assessing the concentration of SARS-CoV-2 RNA in air samples to support risk assessment, especially in indoor environments. Debates on the airborne transmission route of SARS-CoV-2 have been complicated because, among the studies testing the presence of the virus in the air, the percentage of positive samples has often been very low. In the present study, we report preliminary results on a study for the evaluation of parameters that can influence SARS-CoV-2 RNA recovery from quartz fiber filters spotted either by standard single-stranded SARS-CoV-2 RNA or by inactivated SARS-CoV-2 virions. The analytes were spiked on filters and underwent an active or passive sampling; then, they were preserved at −80 °C for different numbers of days (0 to 54) before extraction and analysis. We found a mean recovery of 2.43%, except for the sample not preserved (0 days) that showed a recovery of 13.51%. We found a relationship between the number of days and the recovery percentage. The results presented show a possible issue that relates to the quartz matrix and SARS-CoV-2 RNA recovery. The results are in accordance with the already published studies that described similar methods for SARS-CoV-2 RNA field sampling and that reported non-detectable concentrations of RNA. These outcomes could be false negatives due to sample preservation conditions. Thus, until further investigation, we suggest, as possible alternatives, to keep the filters: (i) in a sealed container for preservation at 4 °C; and (ii) in a viral transport medium for preservation at a temperature below 0 °C.
    URI
    https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85125014276&origin=inward
    DOI/handle
    http://dx.doi.org/10.3390/atmos13020340
    http://hdl.handle.net/10576/33780
    Collections
    • Biological & Environmental Sciences [‎931‎ items ]
    • COVID-19 Research [‎848‎ items ]

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