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Towards novel Cry toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin
(
Springer-Verlag
, 2017 , Article)
Attempts have been made to express or to merge different Cry proteins in order to enhance toxic effects against various insects. Cry1A proteins of Bacillus thuringiensis form a typical bipyramidal parasporal crystal and ...
Potential of Photorhabdus temperata K122 bioinsecticide in protecting wheat flour against Ephestia kuehniella
(
Elsevier Ltd
, 2013 , Article)
The present study reports investigations on the insecticidal activity of the entomopathogenic bacterium Photorhabdus temperata K122 against the Mediterranean flour moth Ephestia kuehniella. Cultured in the optimized medium, ...
Chemical composition and evaluation of antioxidant and antimicrobial activities of Tunisian Thymelaea hirsuta with special reference to its mode of action
(
Elsevier B.V.
, 2013 , Article)
In the present study, we evaluated in vitro antimicrobial and antioxidant properties of Thymelaea hirsuta extracts as well as its phytochemical composition. Total phenolic and flavonoid contents were highest in acetone and ...
Comparative study of Bacillus thuringiensis Cry1Ia and Cry1Aa delta-endotoxins : activation process and toxicity against Prays oleae
(
Elsevier Inc.
, 2010 , Article)
Cry1Ia and Cry1Aa proteins exhibited toxicities against Prays oleae with LC(50) of 189 and 116 ng/cm(2), respectively. The ability to process Cry1Ia11 protoxin by trypsin, chymotrypsin and P. oleae larvae proteases was ...
Amino acids Y229 and F603 are involved in Bacillus thuringiensis Cry1Ac delta-endotoxin stability and toxicity
(
Blackwell Publishing
, 2012 , Article)
Bacillus thuringiensis Cry1Ac toxin shares structurally five conserved blocs with the other δ-endotoxins. To study the role of some amino acids belonging to these regions, two mutations, Y(229) P and F(603) S, were introduced ...
Toxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of Bacillus thuringiensis crystal protein Cry2Aa
(
Wiley
, 2016 , Article)
BACKGROUND
Bacillus thuringiensis δ-endotoxins are the most widely used biopesticides for controlling economically important crop pests and disease vectors. Improving their efficacy is of great benefit. Here, an improvement ...
Susceptibility of Agrotis segetum (noctuidae) to Bacillus thuringiensis and analysis of midgut proteinases
(
Taylor & Francis
, 2015 , Article)
Seventy-eight Bacillus thuringiensis isolates were selected for a screening against the Lepidoptera species Agrotis segetum to search the higher insecticidal activity. In a preliminary bioassay, the spore-crystal mixture ...
Characterisation of novel Bacillus thuringiensis isolates against Aedes aegypti (Diptera: Culicidae) and Ceratitis capitata (Diptera:Tephridae)
(
Elsevier Inc.
, 2015 , Article)
Bacillus thuringiensis is successfully used in pest management strategies as an eco-friendly bioinsecticide. Isolation and identification of new strains with a wide variety of target pests is an ever growing field. In this ...
New Bacillus thuringiensis toxin combinations for biological control of Lepidopteran larvae
(
Elsevier B.V.
, 2014 , Article)
Cyt1Aa from Bacillus thuringiensis israelensis is known by its synergistical activity with B. thuringiensis and Bacillus sphaericus toxins. It is able to improve dipteran specific toxins activity and can prevent or overcome ...
Construction of a Bacillus thuringiensis genetically-engineered strain harbouring the secreted Cry1Ia delta-endotoxin in its crystal
(
Springer Netherlands
, 2011 , Article)
Unlike other Bacillus thuringiensis Cry proteins, Cry1 Ia does not form a crystal since it is a secreted delta-endotoxin. We have engineered a Cry1 Iac chimeric protein by substituting the C-terminal part of Cry1 Ia by the ...