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المؤلفUsman, Kamal
المؤلفSouchelnytskyi, Serhiy
المؤلفAl-Ghouti, Mohammad A.
المؤلفZouari, Nabil
المؤلفAbu-Dieyeh, Mohammed H.
تاريخ الإتاحة2022-12-12T11:10:00Z
تاريخ النشر2022-10-20
اسم المنشورFrontiers in Plant Science
المعرّفhttp://dx.doi.org/10.3389/fpls.2022.1009756
الاقتباسUsman K, Souchelnytskyi S, Al-Ghouti MA, Zouari N and Abu-Dieyeh MH (2022) Proteomic analysis of T. qataranse exposed to lead (Pb) stress reveal new proteins with potential roles in Pb tolerance and detoxification mechanism. Front. Plant Sci. 13:1009756. doi: 10.3389/fpls.2022.1009756
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85141140907&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/37166
الملخصSoil lead (Pb) contamination is one of the environmental problems facing the modern world. Sources of Pb in soil include industrial activities such as mining and smelting processes, agricultural activities such as application of insecticide and municipal sewage sludges, and urban activities such as use of lead in gasoline, paints, and other materials. Phytoremediation is the direct use of living green plants and is an effective, cheap, non-invasive, and environmentally friendly technique used to transfer or stabilize all the toxic metals and environmental pollutants in polluted soil or groundwater. Current work in this area is invested in elucidating mechanisms that underpin toxic-metal tolerance and detoxification mechanisms. The present study aims to gain insight into the mechanisms of Pb tolerance in T. qataranse by comparative proteomics. MALDI-TOF/MS and in silico proteome analysis showed differential protein expression between treated (50 mg kg⎯1 Pb) and untreated (0 mg kg⎯1 Pb) T. qataranse. A total of eighty-six (86) differentially expressed proteins, most of which function in ion and protein binding, antioxidant activity, transport, and abiotic response stress, were identified. In addition, essential stress-regulating metabolic pathways, including glutathione metabolism, cellular response to stress, and regulation of HSF1-mediated heat shock response, were also enriched. Also, at 52- and 49-kDa MW band areas, up to six hypothetical proteins with unknown functions were identified. Of these, protein AXX17_AT2G26660 is highly rich in glycine amino acid residues (up to 76%), suggesting that it is a probable glycine-rich protein (GRP) member. Although GRPs are known to be involved in plant defense against abiotic stress, including salinity and drought, there is no report on their role on Pb tolerance and or detoxification in plants. Further enrichment analysis in the current study reveals that the hypothetical proteins do not interact with known proteins and are not part of any enriched pathway. However, additional research is needed to functionally validate the role of the identified proteins in Pb detoxification mechanism.
راعي المشروعQatar University’s student grant QUST-CAS-SPR-2017-33 supports this study.
اللغةen
الناشرFrontiers Media
الموضوعglycine rich proteins
heavy metals
lead
proteomics
Tetraena qataranse
العنوانProteomic analysis of T. qataranse exposed to lead (Pb) stress reveal new proteins with potential roles in Pb tolerance and detoxification mechanism
النوعArticle
رقم المجلد13
ESSN1664-462X
dc.accessType Open Access


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