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المؤلفZaqout, Sami
المؤلفMannaa, Atef
المؤلفKlein, Oliver
المؤلفKrajewski, Angelika
المؤلفKlose, Joachim
المؤلفLuise-Becker, Lena
المؤلفElsabagh, Ahmed
المؤلفFerih, Khaled
المؤلفKraemer, Nadine
المؤلفRavindran, Ethiraj
المؤلفMakridis, Konstantin
المؤلفKaindl, Angela M.
تاريخ الإتاحة2022-12-25T10:04:20Z
تاريخ النشر2022-01-01
اسم المنشورAnnals of Human Genetics
المعرّفhttp://dx.doi.org/10.1111/ahg.12489
الاقتباسZaqout, S., Mannaa, A., Klein, O., Krajewski, A., Klose, J., Luise-Becker, L., Elsabagh, A., Ferih, K., Kraemer, N., Ravindran, E., Makridis, K., & Kaindl, A. M. (2022). Proteome changes in autosomal recessive primary microcephaly. Annals of Human Genetics, 00, 1– 13. https://doi.org/10.1111/ahg.12489
الرقم المعياري الدولي للكتاب00034800
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85143204256&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/37564
الملخصBackground/aim: : Autosomal recessive primary microcephaly (MCPH) is a rare and genetically heterogeneous group of disorders characterized by intellectual disability and microcephaly at birth, classically without further organ involvement. MCPH3 is caused by biallelic variants in the cyclin-dependent kinase 5 regulatory subunit-associated protein 2 gene CDK5RAP2. In the corresponding Cdk5rap2 mutant or Hertwig's anemia mouse model, congenital microcephaly as well as defects in the hematopoietic system, germ cells and eyes have been reported. The reduction in brain volume, particularly affecting gray matter, has been attributed mainly to disturbances in the proliferation and survival of early neuronal progenitors. In addition, defects in dendritic development and synaptogenesis exist that affect the excitation-inhibition balance. Here, we studied proteomic changes in cerebral cortices of Cdk5rap2 mutant mice. Material and methods: : We used large-gel two-dimensional gel (2-DE) electrophoresis to separate cortical proteins. 2-DE gels were visualized by a trained observer on a light box. Spot changes were considered with respect to presence/absence, quantitative variation and altered mobility. Result: : We identified a reduction in more than 30 proteins that play a role in processes such as cell cytoskeleton dynamics, cell cycle progression, ciliary functions and apoptosis. These proteome changes in the MCPH3 model can be associated with various functional and morphological alterations of the developing brain. Conclusion: : Our results shed light on potential protein candidates for the disease-associated phenotype reported in MCPH3.
اللغةen
الناشرWiley
الموضوعbrain
Cdk5rap2
MCPH
microcephaly
proteome
العنوانProteome changes in autosomal recessive primary microcephaly
النوعArticle
ESSN1469-1809
dc.accessType Open Access


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