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AuthorBagwe, Priyal
AuthorBajaj, Lotika
AuthorGala, Rikhav P.
AuthorD’souza, Martin J.
AuthorZughaier, Susu M.
Available date2023-01-15T05:03:14Z
Publication Date2022-07-01
Publication NameVaccines
Identifierhttp://dx.doi.org/10.3390/vaccines10070983
CitationBagwe, P.; Bajaj, L.; Gala, R.P.; D‘Souza, M.J.; Zughaier, S.M. Assessment of In Vitro Immunostimulatory Activity of an Adjuvanted Whole-Cell Inactivated Neisseria gonorrhoeae Microparticle Vaccine Formulation. Vaccines 2022, 10, 983. https://doi.org/10.3390/vaccines10070983
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85132985508&origin=inward
URIhttp://hdl.handle.net/10576/38318
AbstractThe emergence of drug-resistant gonorrhea infections worldwide combined with the lack of a vaccine is alarming. We prepared a novel microparticulate (MP) vaccine formulation using whole-cell inactivated Neisseria gonorrhoeae as the vaccine antigen, with Alum and AddaVax™ as vaccine adjuvants. The adjuvanted vaccine MP formulation was assessed for in vitro immunostim-ulatory activity, autophagy, and antigen presentation ability. The data shows that the adjuvanted gonococci vaccine MP enhanced autophagy induction in antigen presenting cells (APCs) compared to gonococci vaccine MP without adjuvants, which is important for enhancing antigen presentation. In addition, the adjuvanted vaccine formulation increased the surface expression of antigen presenting molecules MHCI and MHCII as well as co-stimulatory molecules CD40 and CD86 on the surface of dendritic cells. In addition, the gonococci vaccine microparticles at lower doses did not significantly increase the expression of the death receptor CD95 in APCs, which when elevated leads to suboptimal antigen presentation and reduced immune responses. The adjuvanted whole-cell inactivated gonococci microparticle vaccine formulation enhanced antigen uptake, processing, and antigen presentation.
SponsorAn R15 grant from the National Institute of Health funds this project-1R15AI133473-01A1.
Languageen
PublisherMDPI
Subjectantigen presentation
autophagy
gonorrhea
microparticles
Neisseria gonorrhoeae
vaccines
TitleAssessment of In Vitro Immunostimulatory Activity of an Adjuvanted Whole-Cell Inactivated Neisseria gonorrhoeae Microparticle Vaccine Formulation
TypeArticle
Issue Number7
Volume Number10
ESSN2076-393X
dc.accessType Open Access


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