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AuthorBen Hsouna, Anis
AuthorMongi, Saoudi
AuthorCulioli, Gerald
AuthorBlache, Yves
AuthorGhlissi, Zohra
AuthorChaabane, Rim
AuthorEl Feki, Abdelfattah
AuthorJaoua, Samir
AuthorTrigui, Mohamed
Available date2015-12-21T10:39:41Z
Publication Date2013-11
Publication NameToxicology and Industrial Healthen_US
CitationBen Hsouna A, Mongi S, Culioli G, Blache Y, Ghlissi Z, Chaabane R, El Feki A, Jaoua S, Trigui M., "Protective effects of ethyl acetate fraction of Lawsonia inermis fruits extract against carbon tetrachloride-induced oxidative damage in rat liver", Toxicology and Industrial Health, 2013 Nov 8.
ISSN0748-2337
ISSN1477-0393 (online)
URIhttp://dx.doi.org/10.1177/0748233713502839
URIhttp://hdl.handle.net/10576/3969
AbstractThis study aimed to investigate the antioxidant properties of different fractions obtained from the fruits of Lawsonia inermis, a widely used medicinal plant, against carbon tetrachloride (CCl4)-induced oxidative stress in rat liver. The results show that several fractions obtained from L. inermis fruits possessed important antioxidant activity. Among them, the ethyl acetate (EA) fraction showed the highest antioxidant activity. Then, EA fraction was selected for the purification of potential antioxidant compounds. The hepatoprotective effects of EA fraction and its most active constituent, gallic acid (GA), were evaluated against CCl4-induced hepatotoxicity in rats. CCl4 induced oxidative stress by a significant rise in serum marker enzymes. However, pretreatment of rats with EA fraction of fruits of L. inermis at a dose of 250 mg kg-1 body weight and GA significantly lowered some serum biochemical parameters (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase) in treated rats. A significant reduction in hepatic thiobarbituric acid reactive substances and an increase in antioxidant enzymes namely superoxide dismutase, catalase, and glutathione peroxidase by treatment with plant extract and GA, against CCl4-treated rats, were observed. Histopathological examinations showed extensive liver injuries, characterized by extensive hepatocellular necrosis, vacuolization, and inflammatory cell infiltration. This potential antioxidant activity is comparable to those of the major purified antioxidant compound, GA. Based on these results, it was observed that fruits of L. inermis protect liver from oxidative stress induced by CCl4 and thus help in evaluation of traditional claim on this plant.
SponsorTunisian Ministry of Higher Education
Languageen
PublisherSAGE Publications
SubjectLawsonia inermis
SubjectHepato toxicity
SubjectCarbon tetrachloride
SubjectOxidative stress
SubjectBeneficial effect
TitleProtective effects of ethyl acetate fraction of Lawsonia inermis fruits extract against carbon tetrachloride-induced oxidative damage in rat liver
TypeArticle


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