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AuthorPajović, Jelena D.
AuthorDojčilović, Radovan
AuthorBožanić, Dušan K.
AuthorKaščáková, Slavka
AuthorRéfrégiers, Matthieu
AuthorDimitrijević-Branković, Suzana
AuthorVodnik, Vesna V.
AuthorMilosavljević, Aleksandar R.
AuthorPiscopiello, Emanuela
AuthorLuyt, Adriaan S.
AuthorDjoković, Vladimir
Available date2015-12-29T12:54:36Z
Publication Date2015-11
Publication NameColloids and Surfaces B: Biointerfaces
CitationPajovic, J.D., Dojcilovic, R., Bozanic, D.K., Kascakova, S., Refregiers, M., Dimitrijevic-Brankovic, S., Vodnik V.V., Milosavljevic, A.R., Piscopiello E., Luyt A.S., Djokovic, V., Tryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells, (2015) Colloids and Surfaces B: Biointerfaces, 135, article no. 7337, pp. 742-750
AbstractBiocompatible fluorescent nanostructures were prepared by a functionalization of gold nanoparticles with the amino acid tryptophan. The gold-tryptophan bioconjugates were investigated by TEM and HRTEM and various spectroscopy methods (XPS, FTIR, UV-vis and photoluminescence). It was found that the gold nanoparticles, initially 8. nm in diameter, aggregate in the presence of the amino acid. From the XPS and FTIR spectroscopy results, it was concluded that the tryptophan gold interactions mainly take place via indole and carboxyl groups. Although the indole group is involved in the interaction with the gold surfaces, the tryptophan-gold hybrids showed strong fluorescence due to the presence of multilayers of tryptophan. Deep ultra violet (DUV) imaging performed at the SOLEIL synchrotron showed that it is possible to detect these hybrid nanostructures within Escherichia coli cells.
SponsorMinistry of Education and Science of the Republic of Serbia (Project Nos. 172056, 45020, and 171020).
SubjectE. coli
SubjectFluorescence imaging
SubjectGold nanoparticles
TitleTryptophan-functionalized gold nanoparticles for deep UV imaging of microbial cells
Volume Number135

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