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المؤلفKashir, Junaid
المؤلفMistry, Bhavesh V.
المؤلفBuSaleh, Lujain
المؤلفNomikos, Michail
المؤلفAlmuqayyil, Sarah
المؤلفAbu-Dawud, Raed
المؤلفAlYacoub, Nadya
المؤلفHamdan, Hamdan
المؤلفAlHassan, Saad
المؤلفLai, F. Anthony
المؤلفAssiri, Abdullah M.
المؤلفCoskun, Serdar
تاريخ الإتاحة2023-03-27T10:57:02Z
تاريخ النشر2023-02-01
اسم المنشورPharmaceuticals
المعرّفhttp://dx.doi.org/10.3390/ph16020198
الاقتباسKashir, J.; Mistry, B.V.; BuSaleh, L.; Nomikos, M.; Almuqayyil, S.; Abu-Dawud, R.; AlYacoub, N.; Hamdan, H.; AlHassan, S.; Lai, F.A.; Assiri, A.M.; Coskun, S. Antigen Unmasking Is Required to Clinically Assess Levels and Localisation Patterns of Phospholipase C Zeta in Human Sperm. Pharmaceuticals 2023, 16, 198. https://doi.org/10.3390/ph16020198
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85148957685&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/41357
الملخصMammalian oocyte activation is initiated by intracellular calcium (Ca2+) oscillations, driven by the testis-specific phospholipase C zeta (PLCζ). Sperm PLCζ analysis represents a diagnostic measure of sperm fertilisation capacity. The application of antigen unmasking/retrieval (AUM) generally enhanced the visualisation efficacy of PLCζ in mammalian sperm, but differentially affected the PLCζ profiles in sperm from different human males. It is unclear whether AUM affects the diagnosis of PLCζ in human sperm. Herein, we examined whether the application of AUM affected the correlation of PLCζ profiles with sperm parameters and fertilisation capacity. PLCζ fluorescence levels and localisation patterns were examined within the sperm of males undergoing fertility treatment (55 patients aged 29–53) using immunofluorescence in the absence/presence of AUM. The changes in PLCζ profiles following AUM were examined in relation to sperm health and fertilisation outcome. AUM enhanced the observable levels and specific localisation patterns of PLCζ in relation to both optimal sperm parameters and fertilisation outcome, without which significant differences were not observed. The extent of the change in levels and localisation ratios of PLCζ was also affected to a larger degree in terms of the optimal parameters of sperm fertility and fertilisation capacity by AUM. Collectively, AUM was essential to accurately assesses PLCζ in human sperm in both scientific and clinical contexts.
راعي المشروعThis study was supported by a Healthcare Research Fellowship Award (HF-14-16) made by Health and Care Research Wales (HCRW) to J.K., alongside a National Science, Technology, and Innovation plan (NSTIP) project grant (15-MED4186-20) awarded by the King Abdulaziz City for Science and Technology (KACST) made to J.K. and A.M.A.
اللغةen
الناشرMDPI
الموضوعantigen retrieval/unmasking
fertilisation
male infertility
oocyte activation
phospholipase C zeta (PLCzeta)
sperm
العنوانAntigen Unmasking Is Required to Clinically Assess Levels and Localisation Patterns of Phospholipase C Zeta in Human Sperm
النوعArticle
رقم العدد2
رقم المجلد16
ESSN1424-8247
dc.accessType Open Access


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