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AuthorKashir, Junaid
AuthorMistry, Bhavesh V.
AuthorBuSaleh, Lujain
AuthorNomikos, Michail
AuthorAlmuqayyil, Sarah
AuthorAbu-Dawud, Raed
AuthorAlYacoub, Nadya
AuthorHamdan, Hamdan
AuthorAlHassan, Saad
AuthorLai, F. Anthony
AuthorAssiri, Abdullah M.
AuthorCoskun, Serdar
Available date2023-03-27T10:57:02Z
Publication Date2023-02-01
Publication NamePharmaceuticals
Identifierhttp://dx.doi.org/10.3390/ph16020198
CitationKashir, J.; Mistry, B.V.; BuSaleh, L.; Nomikos, M.; Almuqayyil, S.; Abu-Dawud, R.; AlYacoub, N.; Hamdan, H.; AlHassan, S.; Lai, F.A.; Assiri, A.M.; Coskun, S. Antigen Unmasking Is Required to Clinically Assess Levels and Localisation Patterns of Phospholipase C Zeta in Human Sperm. Pharmaceuticals 2023, 16, 198. https://doi.org/10.3390/ph16020198
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85148957685&origin=inward
URIhttp://hdl.handle.net/10576/41357
AbstractMammalian oocyte activation is initiated by intracellular calcium (Ca2+) oscillations, driven by the testis-specific phospholipase C zeta (PLCζ). Sperm PLCζ analysis represents a diagnostic measure of sperm fertilisation capacity. The application of antigen unmasking/retrieval (AUM) generally enhanced the visualisation efficacy of PLCζ in mammalian sperm, but differentially affected the PLCζ profiles in sperm from different human males. It is unclear whether AUM affects the diagnosis of PLCζ in human sperm. Herein, we examined whether the application of AUM affected the correlation of PLCζ profiles with sperm parameters and fertilisation capacity. PLCζ fluorescence levels and localisation patterns were examined within the sperm of males undergoing fertility treatment (55 patients aged 29–53) using immunofluorescence in the absence/presence of AUM. The changes in PLCζ profiles following AUM were examined in relation to sperm health and fertilisation outcome. AUM enhanced the observable levels and specific localisation patterns of PLCζ in relation to both optimal sperm parameters and fertilisation outcome, without which significant differences were not observed. The extent of the change in levels and localisation ratios of PLCζ was also affected to a larger degree in terms of the optimal parameters of sperm fertility and fertilisation capacity by AUM. Collectively, AUM was essential to accurately assesses PLCζ in human sperm in both scientific and clinical contexts.
SponsorThis study was supported by a Healthcare Research Fellowship Award (HF-14-16) made by Health and Care Research Wales (HCRW) to J.K., alongside a National Science, Technology, and Innovation plan (NSTIP) project grant (15-MED4186-20) awarded by the King Abdulaziz City for Science and Technology (KACST) made to J.K. and A.M.A.
Languageen
PublisherMDPI
Subjectantigen retrieval/unmasking
fertilisation
male infertility
oocyte activation
phospholipase C zeta (PLCzeta)
sperm
TitleAntigen Unmasking Is Required to Clinically Assess Levels and Localisation Patterns of Phospholipase C Zeta in Human Sperm
TypeArticle
Issue Number2
Volume Number16
ESSN1424-8247
dc.accessType Open Access


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