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المؤلفVijayanand, Sharon
المؤلفPatil, Smital
المؤلفMenon, Ipshita
المؤلفBraz Gomes, Keegan
المؤلفKale, Akanksha
المؤلفBagwe, Priyal
المؤلفUddin, Mohammad N.
المؤلفZughaier, Susu M.
المؤلفD’Souza, Martin J.
تاريخ الإتاحة2023-05-03T09:27:46Z
تاريخ النشر2023-03-09
اسم المنشورPharmaceutics
المعرّفhttp://dx.doi.org/10.3390/pharmaceutics15030895
الاقتباسVijayanand, S., Patil, S., Menon, I., Braz Gomes, K., Kale, A., Bagwe, P., ... & D’Souza, M. J. (2023). An Adjuvanted Inactivated SARS-CoV-2 Microparticulate Vaccine Delivered Using Microneedles Induces a Robust Immune Response in Vaccinated Mice. Pharmaceutics, 15(3), 895.
الرقم المعياري الدولي للكتاب1999-4923
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85151379093&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/42273
الملخصSARS-CoV-2, the causal agent of COVID-19, is a contagious respiratory virus that frequently mutates, giving rise to variant strains and leading to reduced vaccine efficacy against the variants. Frequent vaccination against the emerging variants may be necessary; thus, an efficient vaccination system is needed. A microneedle (MN) vaccine delivery system is non-invasive, patient-friendly, and can be self-administered. Here, we tested the immune response produced by an adjuvanted inactivated SARS-CoV-2 microparticulate vaccine administered via the transdermal route using a dissolving MN. The inactivated SARS-CoV-2 vaccine antigen and adjuvants (Alhydrogel® and AddaVax™) were encapsulated in poly(lactic-co-glycolic acid) (PLGA) polymer matrices. The resulting MP were approximately 910 nm in size, with a high percentage yield and percent encapsulation efficiency of 90.4%. In vitro, the vaccine MP was non-cytotoxic and increased the immunostimulatory activity measured as nitric oxide release from dendritic cells. The adjuvant MP potentiated the immune response of the vaccine MP in vitro. In vivo, the adjuvanted SARS-CoV-2 MP vaccine induced high levels of IgM, IgG, IgA, IgG1, and IgG2a antibodies and CD4+ and CD8+ T-cell responses in immunized mice. In conclusion, the adjuvanted inactivated SARS-CoV-2 MP vaccine delivered using MN induced a robust immune response in vaccinated mice.
اللغةen
الناشرMultidisciplinary Digital Publishing Institute (MDPI)
الموضوعantibody response
cytotoxicity
immunogenicity
microneedles
microparticles
SARS-CoV-2
T-cell response
العنوانAn Adjuvanted Inactivated SARS-CoV-2 Microparticulate Vaccine Delivered Using Microneedles Induces a Robust Immune Response in Vaccinated Mice
النوعArticle
رقم العدد3
رقم المجلد15
ESSN1999-4923
dc.accessType Open Access


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