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AuthorSalloum-Asfar, Salam
AuthorAbdulla, Sara A.
AuthorTaha, Rowaida Z.
AuthorThompson, I. Richard
AuthorEmara, Mohamed M.
Available date2023-07-06T11:17:16Z
Publication Date2022-11-29
Publication NameCells
Identifierhttp://dx.doi.org/10.3390/cells11233833
CitationSalloum-Asfar, S., Abdulla, S. A., Taha, R. Z., Thompson, I. R., & Emara, M. M. (2022). Combined Noncoding RNA-mRNA Regulomics Signature in Reprogramming and Pluripotency in iPSCs. Cells, 11(23), 3833.
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85143605875&origin=inward
URIhttp://hdl.handle.net/10576/45126
AbstractSomatic cells are reprogrammed with reprogramming factors to generate induced pluripotent stem cells (iPSCs), offering a promising future for disease modeling and treatment by overcoming the limitations of embryonic stem cells. However, this process remains inefficient since only a small percentage of transfected cells can undergo full reprogramming. Introducing miRNAs, such as miR-294 and miR302/3667, with reprogramming factors, has shown to increase iPSC colony formation. Previously, we identified five transcription factors, GBX2, NANOGP8, SP8, PEG3, and ZIC1, which may boost iPSC generation. In this study, we performed quantitative miRNAome and small RNA-seq sequencing and applied our previously identified transcriptome to identify the potential miRNA–mRNA regulomics and regulatory network of other ncRNAs. From each fibroblast (N = 4), three iPSC clones were examined (N = 12). iPSCs and original fibroblasts expressed miRNA clusters differently and miRNA clusters were compared to mRNA hits. Moreover, miRNA, piRNA, and snoRNAs expression profiles in iPSCs and original fibroblasts were assessed to identify the potential role of ncRNAs in enhancing iPSC generation, pluripotency, and differentiation. Decreased levels of let-7a-5p showed an increase of SP8 as described previously. Remarkably, the targets of identifier miRNAs were grouped into pluripotency canonical pathways, on stemness, cellular development, growth and proliferation, cellular assembly, and organization of iPSCs.
SponsorThis research was funded by Qatar Biomedical Research Institute’s (QBRI) internal grant number: QB16.
Languageen
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
Subjectdifferentiation
iPSCs
miRNAs
noncoding RNAs
piRNAs
pluripotency
reprogramming
snoRNAs
TitleCombined Noncoding RNA-mRNA Regulomics Signature in Reprogramming and Pluripotency in iPSCs
TypeArticle
Issue Number23
Volume Number11
ESSN2073-4409
dc.accessType Open Access


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