Platelet–Neutrophil Association in NETs-Rich Areas in the Retrieved AIS Patient Thrombi
المؤلف | Pir, Ghulam Jeelani |
المؤلف | Parray, Aijaz |
المؤلف | Ayadathil, Raheem |
المؤلف | Pananchikkal, Sajitha V. |
المؤلف | Mir, Fayaz Ahmad |
المؤلف | Muhammad, Islam |
المؤلف | Abubakar, Ahmed |
المؤلف | Amir, Nueman |
المؤلف | Hussain, Sohail |
المؤلف | Haroon, Khawaja H. |
المؤلف | Muhammad, Ahmad |
المؤلف | Imam, Yahya |
المؤلف | Patro, Satya Narayana |
المؤلف | Akhtar, Naveed |
المؤلف | Zakaria, Aymen |
المؤلف | Kamran, Saadat |
تاريخ الإتاحة | 2023-07-06T11:22:49Z |
تاريخ النشر | 2022-11-21 |
اسم المنشور | International Journal of Molecular Sciences |
المعرّف | http://dx.doi.org/10.3390/ijms232214477 |
الاقتباس | Pir, G. J., Parray, A., Ayadathil, R., Pananchikkal, S. V., Mir, F. A., Muhammad, I., ... & Kamran, S. (2022). Platelet–Neutrophil Association in NETs-Rich Areas in the Retrieved AIS Patient Thrombi. International Journal of Molecular Sciences, 23(22), 14477. |
الرقم المعياري الدولي للكتاب | 1661-6596 |
الملخص | Histological structure of thrombi is a strong determinant of the outcome of vascular recanalization therapy, the only treatment option for acute ischemic stroke (AIS) patients. A total of 21 AIS patients from this study after undergoing non-enhanced CT scan and multimodal MRI were treated with mechanical stent-based and manual aspiration thrombectomy, and thromboembolic retrieved from a cerebral artery. Complementary histopathological and imaging analyses were performed to understand their composition with a specific focus on fibrin, von Willebrand factor, and neutrophil extracellular traps (NETs). Though distinct RBC-rich and platelet-rich areas were found, AIS patient thrombi were overwhelmingly platelet-rich, with 90% of thrombi containing <40% total RBC-rich contents (1.5 to 37%). Structurally, RBC-rich areas were simple, consisting of tightly packed RBCs in thin fibrin meshwork with sparsely populated nucleated cells and lacked any substantial von Willebrand factor (VWF). Platelet-rich areas were structurally more complex with thick fibrin meshwork associated with VWF. Plenty of leukocytes populated the platelet-rich areas, particularly in the periphery and border areas between platelet-rich and RBC-rich areas. Platelet-rich areas showed abundant activated neutrophils (myeloperoxidase+ and neutrophil-elastase+) containing citrullinated histone-decorated DNA. Citrullinated histone-decorated DNA also accumulated extracellularly, pointing to NETosis by the activated neutrophils. Notably, NETs-containing areas showed strong reactivity to VWF, platelets, and high-mobility group box 1 (HMGB1), signifying a close interplay between these components. |
راعي المشروع | This research was funded by a grant awarded by Medical Research Council (MRC), Hamad Medical Corporation (HMC) (grant number IRGC-02-SI-029). |
اللغة | en |
الناشر | Multidisciplinary Digital Publishing Institute (MDPI) |
الموضوع | AIS fibrin HMGB1 leukocytes NETs neutrophils platelets stroke t-PA von Willebrand factor |
النوع | Article |
رقم العدد | 22 |
رقم المجلد | 23 |
ESSN | 1422-0067 |
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