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المؤلفPir, Ghulam Jeelani
المؤلفParray, Aijaz
المؤلفAyadathil, Raheem
المؤلفPananchikkal, Sajitha V.
المؤلفMir, Fayaz Ahmad
المؤلفMuhammad, Islam
المؤلفAbubakar, Ahmed
المؤلفAmir, Nueman
المؤلفHussain, Sohail
المؤلفHaroon, Khawaja H.
المؤلفMuhammad, Ahmad
المؤلفImam, Yahya
المؤلفPatro, Satya Narayana
المؤلفAkhtar, Naveed
المؤلفZakaria, Aymen
المؤلفKamran, Saadat
تاريخ الإتاحة2023-07-06T11:22:49Z
تاريخ النشر2022-11-21
اسم المنشورInternational Journal of Molecular Sciences
المعرّفhttp://dx.doi.org/10.3390/ijms232214477
الاقتباسPir, G. J., Parray, A., Ayadathil, R., Pananchikkal, S. V., Mir, F. A., Muhammad, I., ... & Kamran, S. (2022). Platelet–Neutrophil Association in NETs-Rich Areas in the Retrieved AIS Patient Thrombi. International Journal of Molecular Sciences, 23(22), 14477.
الرقم المعياري الدولي للكتاب1661-6596
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85142806490&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/45128
الملخصHistological structure of thrombi is a strong determinant of the outcome of vascular recanalization therapy, the only treatment option for acute ischemic stroke (AIS) patients. A total of 21 AIS patients from this study after undergoing non-enhanced CT scan and multimodal MRI were treated with mechanical stent-based and manual aspiration thrombectomy, and thromboembolic retrieved from a cerebral artery. Complementary histopathological and imaging analyses were performed to understand their composition with a specific focus on fibrin, von Willebrand factor, and neutrophil extracellular traps (NETs). Though distinct RBC-rich and platelet-rich areas were found, AIS patient thrombi were overwhelmingly platelet-rich, with 90% of thrombi containing <40% total RBC-rich contents (1.5 to 37%). Structurally, RBC-rich areas were simple, consisting of tightly packed RBCs in thin fibrin meshwork with sparsely populated nucleated cells and lacked any substantial von Willebrand factor (VWF). Platelet-rich areas were structurally more complex with thick fibrin meshwork associated with VWF. Plenty of leukocytes populated the platelet-rich areas, particularly in the periphery and border areas between platelet-rich and RBC-rich areas. Platelet-rich areas showed abundant activated neutrophils (myeloperoxidase+ and neutrophil-elastase+) containing citrullinated histone-decorated DNA. Citrullinated histone-decorated DNA also accumulated extracellularly, pointing to NETosis by the activated neutrophils. Notably, NETs-containing areas showed strong reactivity to VWF, platelets, and high-mobility group box 1 (HMGB1), signifying a close interplay between these components.
راعي المشروعThis research was funded by a grant awarded by Medical Research Council (MRC), Hamad Medical Corporation (HMC) (grant number IRGC-02-SI-029).
اللغةen
الناشرMultidisciplinary Digital Publishing Institute (MDPI)
الموضوعAIS
fibrin
HMGB1
leukocytes
NETs
neutrophils
platelets
stroke
t-PA
von Willebrand factor
العنوانPlatelet–Neutrophil Association in NETs-Rich Areas in the Retrieved AIS Patient Thrombi
النوعArticle
رقم العدد22
رقم المجلد23
ESSN1422-0067
dc.accessType Open Access


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