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AuthorKobeissy, Firas
AuthorShaito, Abdullah
AuthorKaplan, Abdullah
AuthorBaki, Lama
AuthorHayek, Hassan
AuthorDagher-Hamalian, Carole
AuthorNehme, Ali
AuthorGhali, Rana
AuthorAbidi, Emna
AuthorHusari, Ahmad
AuthorZeidan, Asad
AuthorZouein, Fouad A.
AuthorZibara, Kazem
Available date2023-09-06T05:27:58Z
Publication Date2017
Publication NameOxidative Medicine and Cellular Longevity
ResourceScopus
ISSN19420900
URIhttp://dx.doi.org/10.1155/2017/5135241
URIhttp://hdl.handle.net/10576/47270
AbstractCigarette smoking (S) is a risk factor for progressive chronic kidney disease, renal dysfunction, and renal failure. In this study, the effect of smoking on kidney function was investigated in a mouse model of myocardial infarction (MI) using 4 groups: control (C), smoking (S), MI, and S+MI. Histological analysis of S+MI group showed alterations in kidney structure including swelling of the proximal convoluted tubules (PCTs), thinning of the epithelial lining, focal loss of the brush border of PCTs, and patchy glomerular retraction. Molecular analysis revealed that nephrin expression was significantly reduced in the S+MI group, whereas sodium-hydrogen exchanger-1 (NHE-1) was significantly increased, suggesting altered glomerular filtration and kidney functions. Moreover, S+MI group, but not S alone, showed a significant increase in the expression of connective tissue growth factor (CTGF) and fibrotic proteins fibronectin (FN) and α-smooth muscle actin (SMA), in comparison to controls, in addition to a significant increase in mRNA levels of IL-6 and TNF-α inflammatory markers. Finally, reactive oxygen species (ROS) production was significantly accentuated in S+MI group concomitant with a significant increase in NOX-4 protein levels. In conclusion, smoking aggravates murine acute renal damage caused by MI at the structural and molecular levels by exacerbating renal dysfunction.
SponsorThis work was supported by grants from the Medical Practice Plan (MPP) at AUB (grant title "Effect of Second Hand Smoking (SHS) on Cardiac and Vascular Smooth Muscle Remodeling: A Targeted and Global Approach." Lead PI: Firas Kobeissy, co-PIs: Asad Zeidan and Ahmad Husari), from Lebanese National Council for Scientific Research (Kazem Zibara), from AUB URB (Firas Kobeissy), and from Lebanese University grant (Kazem Zibara).
Languageen
PublisherHindawi Limited
SubjectAnimals
Cigarette Smoking
Disease Models, Animal
Kidney
Male
Mice
Mice, Inbred C57BL
Myocardial Infarction
Renal Insufficiency, Chronic
TitleAcute exposure to cigarette smoking followed by myocardial infarction aggravates renal damage in an in vivo mouse model
TypeArticle
Volume Number2017
dc.accessType Open Access


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