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AuthorMokni-Tlili, Sonia
AuthorMehri, Inès
AuthorGhorbel, Manel
AuthorHassen, Wafa
AuthorHassen, Abdennaceur
AuthorJedidi, Naceur
AuthorHamdi, Helmi
Available date2023-10-11T10:47:14Z
Publication Date2020-12-01
Publication NameArchives of Microbiology
Identifierhttp://dx.doi.org/10.1007/s00203-020-01935-7
CitationMokni-Tlili, S., Mehri, I., Ghorbel, M. et al. Community-level genetic profiles of actinomycetales in long-term biowaste-amended soils. Arch Microbiol 202, 2607–2617 (2020). https://doi.org/10.1007/s00203-020-01935-7
ISSN03028933
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85088256981&origin=inward
URIhttp://hdl.handle.net/10576/48452
AbstractActinomycetales is an order of actinobacteria that have an important role in the decomposition of organic matter. Their abundance and distribution can reflect a good level of soil fertility as well as biological activity. In this research study, actinomycetal diversity in soil was investigated under various field treatments with biowastes. Initially, unvegetated agricultural soil plots of 4 m2 had been annually amended with increasing rates of municipal solid waste compost (MSWC at 40, 80 and 120 t ha−1 year−1) and farmyard manure (FM at 40 and 120 t ha−1 year−1) for eight consecutive years. Control consisted of unamended soil and all treatments were distributed in four randomized complete blocks. At the end of the experimental period, total DNA was extracted from fresh topsoil samples (0–20 cm) then nested PCR-DGGE sequencing method was applied to assess the long-term effect of treatments on the diversity of actinomycetes. Analytical outcomes revealed the presence of ten actinomycetal families with Streptomycetaceae, Pseudonocardiaceae and Nocardioidaceae being the most dominant regardless to changes in experimental conditions. Besides, the long-term accumulation of both biowastes in soil affected the diversity of actinomycetal communities in different ways including contribution, stimulation or inhibition. Interestingly, soil treated with MSWC at an equivalent rate of 40 t ha−1 year−1 was likely to provide optimal growth conditions for major identified genera because it showed the highest actinomycetal diversity as compared to the rest of the treatments.
SponsorThis research work was supported by a Grant from the Tunisian Ministry of Higher Education and Scientific Research in the framework of a project entitled “MSW Composting and Agricultural Valorization”.
Languageen
PublisherSpringer
SubjectActinomycetal diversity
Biowastes
Nested PCR-DGGE
Soil amendments
TitleCommunity-level genetic profiles of actinomycetales in long-term biowaste-amended soils
TypeArticle
Pagination2607-2617
Issue Number10
Volume Number202
ESSN1432-072X
dc.accessType Full Text


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