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المؤلفRoberts, Matthew J.
المؤلفRichards, Renee S.
المؤلفChow, Clement W.K.
المؤلفDoi, Suhail A.R.
المؤلفSchirra, Horst Joachim
المؤلفBuck, Marion
المؤلفSamaratunga, Hemamali
المؤلفPerry-Keene, Joanna
المؤلفPayton, Diane
المؤلفYaxley, John
المؤلفLavin, Martin F.
المؤلفGardiner, Robert A.
تاريخ الإتاحة2016-10-12T10:45:31Z
تاريخ النشر2016-09
اسم المنشورProstate International
المعرّفhttp://dx.doi.org/10.1016/j.prnil.2016.04.002
الاقتباسRoberts MJ, Richards RS, Chow CWK, et al. Prostate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers. Prostate International. 2016;4(3):97-102
الرقم المعياري الدولي للكتاب22878882
معرّف المصادر الموحدhttp://www.sciencedirect.com/science/article/pii/S2287888216300022
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4877
الملخصBackground Prostate cancer (PCa) diagnosis requires improvement with the aid of more accurate biomarkers. Postejaculate urethral washings (PEUW) could be a physiological equivalent to urine obtained following rectal prostatic massage, the current basis for the prostate cancer antigen 3 (PCA3) test. The aim of this study was to investigate if PEUW contained prostate-based material, evidenced by the presence of prostate specific antigen (PSA), and to evaluate the diagnostic performance of PEUW-based biomarkers. Methods Male patients referred for elevated serum PSA or abnormal digital rectal examination provided ejaculate and PEUW samples. PSA, PCA3, and β2-microglobulin (β2M) were quantified in ejaculate and PEUW and compared with absolute and clinically significant (according to D’Amico criteria) PCa presence, as determined by biopsies. Diagnostic performance was determined and compared with serum PSA using receiver operating characteristic analysis. Results From 83 patients who provided PEUW samples, paired analysis with ejaculate samples was possible for 38 patients, while analysis in an unpaired, extended cohort was possible for 62 patients. PSA and PCA3 were detected in PEUW, normalized to β2M, and PCA3:PSA was calculated. In predicting absolute PCa status, PCA3:β2M in ejaculate [area under the curve (AUC) 0.717] and PEUW (AUC 0.569) were insignificantly better than PCA3:PSA (AUC 0.668 and 0.431, respectively) and comparable with serum PSA (AUC 0.617) with similar trends observed for the extended cohort. When considering clinically significant PCa presence, serum PSA in the comparison (AUC 0.640) and extended cohorts (AUC 0.665) was comparable with PCA3: β2M (AUC 0.667) and PCA3:PSA (AUC 0.605) in ejaculate, with lower estimates for PEUW in the comparison (PCA3: β2M AUC 0.496; PCA3:PSA AUC 0.342) and extended (PCA3: β2M AUC 0.497; PCA3:PSA AUC 0.469) cohorts. The statistical analysis was limited by sample size. Conclusion PEUW contains prostatic material, but has limited diagnostic accuracy when considering cell-derived DNA analysis. PCA3-based markers in ejaculate are comparable to serum PSA and digital rectal examination–urine markers.
راعي المشروعRoyal Brisbane and Women's Hospital Foundation (Ref: 2014002678), the Prostate Cancer Foundation of Australia and Cancer Council Queensland
اللغةen
الناشرElsevier
الموضوعBiomarker
Ejaculate
mRNA
Prostate Cancer
Prostate Cancer Antigen 3
Prostate Specific Antigen
العنوانProstate-based biofluids for the detection of prostate cancer: A comparative study of the diagnostic performance of cell-sourced RNA biomarkers
النوعArticle
الصفحات97-102
رقم العدد3
رقم المجلد4
Open Access user License http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.accessType Open Access


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