عرض بسيط للتسجيلة

المؤلفBertok, Tomas
المؤلفSediva, Alena
المؤلفFilip, Jaroslav
المؤلفIlcikova, Marketa
المؤلفKasak, Peter
المؤلفVelic, Dusan
المؤلفJane, Eduard
المؤلفMravcová, Martina
المؤلفRovenský, Jozef
المؤلفKunzo, Pavol
المؤلفLobotka, Peter
المؤلفŠmatko, Vasilij
المؤلفVikartovská, Alica
المؤلفTkac, Jan
تاريخ الإتاحة2016-11-13T10:42:33Z
تاريخ النشر2015-06-30
اسم المنشورLangmuir
المعرّفhttp://dx.doi.org/10.1021/acs.langmuir.5b00944
الاقتباسBertok T, Šedivá A, Filip J, et al. Carboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum. Langmuir. 2015;31(25):7148-715.
الرقم المعياري الدولي للكتاب0743-7463
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4991
الملخصImpedimetric lectin biosensors capable of recognizing two different carbohydrates (galactose and sialic acid) in glycans attached to antibodies isolated from human serum were prepared. The first step entailed the modification of a gold surface by a self-assembled monolayer (SAM) deposited from a solution containing a carboxybetaine-terminated thiol applied to the subsequent covalent immobilization of lectins and to resist nonspecific protein adsorption. In the next step, Sambucus nigra agglutinin (SNA) or Ricinus communis agglutinin (RCA) was covalently attached to the SAM, and the whole process of building a bioreceptive layer was optimized and characterized using a diverse range of techniques including electrochemical impedance spectroscopy, cyclic voltammetry, quartz crystal microbalance, contact angle measurements, zeta-potential assays, X-ray photoelectron spectroscopy, and atomic force microscopy. In addition, the application of the SNA-based lectin biosensor in the glycoprofiling of antibodies isolated from the human sera of healthy individuals and of patients suffering from rheumatoid arthritis (RA) was successfully validated using an SNA-based lectin microarray. The results showed that the SNA lectin, in particular, is capable of discriminating between the antibodies isolated from healthy individuals and those from RA patients based on changes in the amount of sialic acid present in the antibodies. In addition, the results obtained by the application of RCA and SNA biosensors indicate that the abundance of galactose and sialic acid in antibodies isolated from healthy individuals is age-related.
راعي المشروعSlovak Scientific Grant Agency VEGA 2/0162/14 and from the Slovak Research and Development Agency APVV 0282-11. The European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement no. 311532. This publication was made possible by NPRP grant no. 6-381-1-078 from the Qatar National Research Fund (a member of the Qatar Foundation).
اللغةen
الناشرAmerican Chemical Society
الموضوعArthritis, Rheumatoid/blood
Electrochemistry
Biosensing Techniques/methods
العنوانCarboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum
النوعArticle
الصفحات7148-7157
رقم العدد25
رقم المجلد31
ESSN1520-5827
dc.accessType Open Access


الملفات في هذه التسجيلة

Thumbnail

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة