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AuthorKlukova, Ludmila
AuthorBertok, Tomas
AuthorPetrikova, Miroslava
AuthorSediva, Alena
AuthorMislovicova, Danica
AuthorKatrlik, Jaroslav
AuthorVikartovska, Alica
AuthorFilip, Jaroslav
AuthorKasak, Peter
AuthorAndicsová-Eckstein, Anita
AuthorMosnáček, Jaroslav
AuthorLukáč, Jozef
AuthorRovenský, Jozef
AuthorImrich, Richard
AuthorTkac, Jan
Available date2016-11-21T11:25:22Z
Publication Date2015-01
Publication NameAnalytica Chimica Actaen_US
CitationLudmila Klukova, Tomas Bertok, Miroslava Petrikova, Alena Sediva, Danica Mislovicova, Jaroslav Katrlik, Alica Vikartovska, Jaroslav Filip, Peter Kasak, Anita Andicsová-Eckstein, Jaroslav Mosnáček, Jozef Lukáč, Jozef Rovenský, Richard Imrich, Jan Tkac, Glycoprofiling as a novel tool in serological assays of systemic sclerosis: A comparative study with three bioanalytical methods, Analytica Chimica Acta, Volume 853, 1 January 2015, Pages 555-562
AbstractSystemic sclerosis (SSc) is an autoimmune disease seriously affecting patient’s quality of life. The heterogeneity of the disease also means that identification and subsequent validation of biomarkers of the disease is quite challenging. A fully validated single biomarker for diagnosis, prognosis, disease activity and assessment of response to therapy is not yet available. The main aim of this study was to apply an alternative assay protocol to the immunoassay-based analysis of this disease by employment of sialic acid recognizing lectin Sambucus nigra agglutinin (SNA) to glycoprofile serum samples. To our best knowledge this is the first study describing direct lectin-based glycoprofiling of serum SSc samples. Three different analytical methods for glycoprofiling of serum samples relying on application of lectins are compared here from a bioanalytical point of view including traditional ELISA-like lectin-based method (ELLA), novel fluorescent lectin microarrays and ultrasensitive impedimetric lectin biosensors. Results obtained by all three bioanalytical methods consistently showed differences in the level of sialic acid present on glycoproteins, when serum from healthy people was compared to the one from patients having SSc. Thus, analysis of sialic acid content in human serum could be of a diagnostic value for future detection of SSc, but further work is needed to enhance selectivity of assays for example by glycoprofiling of a fraction of human serum enriched in antibodies for individual diagnostics.
SponsorSlovak Scientific Grant Agency VEGA 2/0162/14 and from the Slovak Research and Development Agency APVV 0282-11 is acknowledged. The research leading to these results has received funding from the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement No. 311532 and this work has received funding from the European Union’s Seventh Framework Programme for research, Technological Development and Demonstration under grant agreement No. 317420. This publication is the result of the project implementation: Applied Research in the field of Industrial Biocatalysis, ITMS code: 26240220079 supported by the Research & Development Operational Programme funded by the ERDF. Research leading to these results was supported by BASF Slovakia.
SubjectSialic acid
SubjectElectrochemical impedance spectroscopy
SubjectLectin microarray
SubjectEnzyme-linked lectin assay
SubjectSystemic sclerosis
TitleGlycoprofiling as a novel tool in serological assays of systemic sclerosis: A comparative study with three bioanalytical methods
Volume Number853

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