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AuthorNaïja, Azza
AuthorMutlu, Onur
AuthorKhan, Talha
AuthorSeers, Thomas Daniel
AuthorYalcin, Huseyin C
Available date2024-04-24T05:48:52Z
Publication Date2024-04-23
Publication NameBMC Biomedical Engineering
Identifierhttp://dx.doi.org/10.1186/s42490-024-00078-w
CitationNaïja, A., Mutlu, O., Khan, T. et al. An optimized CT-dense agent perfusion and micro-CT imaging protocol for chick embryo developmental stages. BMC biomed eng 6, 3 (2024). https://doi.org/10.1186/s42490-024-00078-w
URIhttp://hdl.handle.net/10576/54155
AbstractCompared to classical techniques of morphological analysis, micro-CT (μ-CT) has become an effective approach allowing rapid screening of morphological changes. In the present work, we aimed to provide an optimized micro-CT dense agent perfusion protocol and μ-CT guidelines for different stages of chick embryo cardiogenesis. Our study was conducted over a period of 10 embryonic days (Hamburger-Hamilton HH36) in chick embryo hearts. During the perfusion of the micro-CT dense agent at different developmental stages (HH19, HH24, HH27, HH29, HH31, HH34, HH35, and HH36), we demonstrated that durations and volumes of the injected contrast agent gradually increased with the heart developmental stages contrary to the flow rate that was unchanged during the whole experiment. Analysis of the CT imaging confirmed the efficiency of the optimized parameters of the heart perfusion.
SponsorThis work was funded by Qatar National Research Fund (QNRF) and National Priority Research Program (Grant No. NPRP 10-0123-170222). Open-access finding is provided by the Qatar National Library. We extend our gratitude to Ms. Maha Elser for her invaluable assistance during the training on the perfusion protocol.
Languageen
PublisherBMC
SubjectCardiogenesis
Chick embryo
Heart morphology
Micro-CT
Microfil
Perfusion
Volume rendering
TitleAn optimized CT-dense agent perfusion and micro-CT imaging protocol for chick embryo developmental stages.
TypeArticle
Issue Number1
Volume Number6
ESSN2524-4426
dc.accessType Open Access


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