Show simple item record

AuthorAbu-Madi, Marawan
AuthorBoughattas, Sonia
AuthorBehnke, Jerzy M
AuthorSharma, Aarti
AuthorIsmail, Ahmed
Available date2017-10-16T09:35:26Z
Publication Date2017-09-06
Publication NameParasites & Vectorsen_US
Identifierhttp://dx.doi.org/10.1186/s13071-017-2346-7
CitationMarawan Abu-Madi, Sonia Boughattas, Jerzy M. Behnke, Aarti Sharma and Ahmed Ismail "Coproscopy and molecular screening for detection of intestinal protozoa" Parasites & Vectors (2017) 10:414
URIhttp://hdl.handle.net/10576/5691
AbstractIntestinal parasitosis is one of several health concerns about immigrants who travel from endemic to non-endemic regions. Reliable rapid sensitive diagnostic tools, for use in non-endemic regions, are urgently required to enable frequent assessment of immigrant workers in jobs where risk of local transmission is a particular concern (e.g. food-handlers). We assessed the burden of intestinal protozoa in newly arrived immigrants and those applying for renewal of work permits in Qatar (n = 735), by both microscopic examination of stool samples and by Real Time PCR methodology. Prevalence was considerably higher using RT-PCR compared with coproscopy (Blastocystis hominis: 65.2 vs 7.6%; Giardia duodenalis: 14.3 vs 2.9%; Entamoeba histolytica: 1.6 vs 1.2%). Dientamoeba fragilis was sought only by RT-PCR (prevalence of 25.4%). Prevalence of G. duodenalis was significantly higher in male subjects, associated with blue collar workers and declined over time. Prevalence of B. hominis varied significantly with region of origin of subjects with highest values recorded among African immigrants. Prevalence of D. fragilis also varied with region of origin of subjects, and was lower in young female subjects and in renewal applicants compared with first-time applicants for work permits. We strongly recommend that, henceforth, intestinal protozoa should be screened by RT-PCR, with a particular focus on frequent assessment of immigrant food-handlers.
SponsorNPRP8–1556–3-313
Languageen
PublisherBioMed Central
SubjectCoproscopy
SubjectFood-handlers
SubjectIntestinal protozoa
SubjectNew immigrant
SubjectQatar
SubjectRT-PCR
SubjectRenewal applicant
TitleCoproscopy and molecular screening for detection of intestinal protozoa
TypeArticle
Issue Number414
Volume Number10
dc.identifier.essn 1756-3305


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record