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المؤلفHume, Michael
المؤلفSohail, Muhammad Umar
تاريخ الإتاحة2018-02-12T07:04:46Z
تاريخ النشر2018-01-01
اسم المنشورFoodborne Pathogens and Disease
المعرّفhttp://dx.doi.org/10.1089/fpd.2017.2365
الاقتباسHume Michael and Sohail Muhammad Umar. Foodborne Pathogens and Disease. January 2018, ahead of print. https://doi.org/10.1089/fpd.2017.2365
الرقم المعياري الدولي للكتاب1535-3141
معرّف المصادر الموحدhttp://hdl.handle.net/10576/6323
الملخصEnteropathogen colonization and product contamination are major poultry industry problems. The emergence of antibiotic resistance, and associated risks to human health, is limiting the use of antibiotics as first-line defense against enteropathogens in poultry. The chitin derivative, chitosan, has drawn substantial attention for its bactericidal properties. Different molecular weight (MW) chitosans can have varied effects against different bacteria in monoculture. In the current study, cecal contents from each of three market-age broilers and Salmonella Typhimurium, as indicator enteropathogen, were exposed to in vitro anaerobic culture to three chitosan preparations (0.08%, wt/vol), low (LMW), medium (MMW), and coarse (CMW). Effects of chitosan and the carrier solvent acetic acid, on cecal bacteria and Salmonella, were examined by denaturing gradient gel electrophoresis (DGGE) and Salmonella enumeration. Bacterial profiles for the three cecal contents were shown by DGGE to be very different. Each of the three cecal contents grown in the presence of 0.08% acetic acid was very different from the same contents grown without the chitosan solvent. Culturing cecal contents in the presence of chitosan altered the bacterial DGGE profiles from the control and acetic acid-only cultures. The DGGE chitosan-treated profiles for all three cecal sources were identical to each other regardless of the MW chitosan in the culture medium. Compared with Salmonella in monoculture, Salmonella decreased (p < 0.05) by about 1.5 log CFU/mL when grown in mixed culture with cecal contents. Salmonella monocultures in the presence of 0.08% of the chitosan solvent acetic acid decreased (p < 0.05) counts by almost 3.5 log CFU/mL. Combining acetic acid and cecal contents reduced (p < 0.05) Salmonella by 7 log CFU/mL. Adding the chitosan preparations to the mixtures reduced (p < 0.05) Salmonella by 8 log CFU/mL.
اللغةen
الناشرMary Ann Liebert
الموضوعChitosan
DGGE
Salmonella
broiler
ceca
العنوانDenaturing Gradient Gel Electrophoresis-Polymerase Chain Reaction Comparison of Chitosan Effects on Anaerobic Cultures of Broiler Cecal Bacteria and Salmonella Typhimurium.
النوعArticle
ESSN1556-7125
dc.accessType Abstract Only


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