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AuthorTavalaee M.
AuthorNomikos M.
AuthorLai F.A.
AuthorNasr-Esfahani M.H.
Available date2020-01-01T10:25:03Z
Publication Date2018
Publication NameReproductive BioMedicine Online
ResourceScopus
ISSN14726483
URIhttp://dx.doi.org/10.1016/j.rbmo.2017.12.013
URIhttp://hdl.handle.net/10576/12455
AbstractGlobozoospermia is characterized by the presence of 100% acrosomeless round-headed spermatozoa in an ejaculate. Failed fertilization after intracytoplasmic sperm injection (ICSI) is commonly reported for globozoospermic couples and can be overcome by artificial oocyte activation (AOA). Phospholipase C zeta (PLC?) is one of the main sperm factors involved in oocyte activation and its low expression levels mainly account for fertilization failure. Deletion of the DPY19L2 gene is reported as a main genetic cause in over 70% of infertile men with globozoospermia. The current study assesses the expression profile of sperm PLC? at RNA and protein levels in 32 DPY19L2 deletion-mediated globozoospermic men and reports corresponding clinical outcomes following ICSI with AOA. The expression of PLC? relative to GAPDH at RNA (0.78 � 0.16 versus 1.65 � 0.24; P = 0.02) and protein (0.39 � 0.12 versus 0.83 � 0.13; P = 0.01) levels in globozoospermic men with DPY19L2 deletion was significantly lower compared with fertile men (n = 32). Fertilization rate in globozoospermic couples following ICSI-AOA was significantly lower compared with fertile men (53.14 � 5.13% versus 87.64 � 2.38%, P < 0.001). However, implantation (26.2%) and pregnancy (53.8%) rates were not jeopardized by DPY19L2 deletion in these couples. - 2017 Reproductive Healthcare Ltd.
SponsorThis study was supported by the Royan Institute ( 94000127 ) and we would like to express our gratitude to the staff of Isfahan Fertility and Infertility for their full support. Mohammad Hossein Nasr-Esfahani received his PhD from the University of Cambridge and is Professor at the Royan Institute, and Laboratory Director of the Isfahan Fertility and Infertility Centre. His special interests are male infertility and animal cloning. In addition, he has over 350 publications in international and national journals. Key message Unlabelled box ICSI-AOA resulted in a high fertilization rate in globozoospermic men with low expression of PLC? and DPY19L2 deletion.
Languageen
PublisherElsevier Ltd
SubjectDPY19L2 deletion
Fertilization
Globozoospermia
ICSI-AOA
PLC?
Pregnancy
TitleExpression of sperm PLC? and clinical outcomes of ICSI-AOA in men affected by globozoospermia due to DPY19L2 deletion
TypeArticle
Pagination348-355
Issue Number3
Volume Number36
dc.accessType Abstract Only


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