عرض بسيط للتسجيلة

المؤلفFardoun M.
المؤلفIratni R.
المؤلفDehaini H.
المؤلفEid A.
المؤلفGhaddar T.
المؤلفEl-Elimat T.
المؤلفAlali F.
المؤلفBadran A.
المؤلفEid A.H.
المؤلفBaydoun E.
تاريخ الإتاحة2020-04-05T10:53:20Z
تاريخ النشر2019
اسم المنشورBiomolecules
المصدرScopus
الرقم المعياري الدولي للكتاب2218273X
معرّف المصادر الموحدhttp://dx.doi.org/10.3390/biom9110716
معرّف المصادر الموحدhttp://hdl.handle.net/10576/13811
الملخصRemodeling of arterioles is a pivotal event in the manifestation of many inflammation-based cardio-vasculopathologies, such as hypertension. During these remodeling events, vascular smooth muscle cells (VSMCs) switch from a contractile to a synthetic phenotype. The latter is characterized by increased proliferation, migration, and invasion. Compounds with anti-inflammatory actions have been successful in attenuating this phenotypic switch. While the vast majority of studies investigating phenotypic modulation were undertaken in VSMCs isolated from large vessels, little is known about the effect of such compounds on phenotypic switch in VSMCs of microvessels (microVSMCs). We have recently characterized a novel homoisoflavonoid that we called 7-O-methylpunctatin (MP). In this study, we show that MP decreased FBS-induced cell proliferation, migration, invasion, and adhesion. MP also attenuated adhesion of THP-1 monocytes to microVSMCs, abolished FBS-induced expression of MMP-2, MMP-9, and NF-?B, as well as reduced activation of ERK1/2 and FAK. Furthermore, MP-treated VSMCs showed an increase in early (myocardin, SM-22?, SM-?) and mid-term (calponin and caldesmon) differentiation markers and a decrease in osteopontin, a protein highly expressed in synthetic VSMCs. MP also reduced transcription of cyclin D1, CDK4 but increased protein levels of p21 and p27. Taken together, these results corroborate an anti-inflammatory action of MP on human microVSMCs. Therefore, by inhibiting the synthetic phenotype of microVSMCs, MP may be a promising modulator for inflammation-induced arteriolar pathophysiology. - 2019 by the authors. Licensee MDPI, Basel, Switzerland.
راعي المشروعFunding: This work was supported by the American University of Beirut (Grant # MPP 320133 to A.E.), University of Petra (Grant #: 5/4/2019) to A.B., E.B., and A.E., and the National Council for Scientific Research (CNRS) to M.F.
اللغةen
الناشرMDPI AG
الموضوع7-O-methylpunctatin
Arterioles
Homoisoflavonoids
Inflammation
Phenotypic switch
Vascular smooth muscle cells
العنوان7-O-methylpunctatin, a novel homoisoflavonoid, inhibits phenotypic switch of human arteriolar smooth muscle cells
النوعArticle
رقم العدد11
رقم المجلد9
dc.accessType Open Access


الملفات في هذه التسجيلة

Thumbnail

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة