Immunofluorescence Staining of Paraffin Sections Step by Step.
المؤلف | Zaqout, Sami |
المؤلف | Becker, Lena-Luise |
المؤلف | Kaindl, Angela M |
تاريخ الإتاحة | 2020-11-30T06:51:50Z |
تاريخ النشر | 2020 |
اسم المنشور | Frontiers in Neuroanatomy |
المعرّف | http://dx.doi.org/10.3389/fnana.2020.582218 |
الاقتباس | Zaqout S, Becker LL, Kaindl AM. Immunofluorescence Staining of Paraffin Sections Step by Step. Front Neuroanat. 2020;14:582218. Published 2020 Nov 9. doi:10.3389/fnana.2020.582218 |
الرقم المعياري الدولي للكتاب | 1662-5129 |
الملخص | Immunofluorescence staining is the most frequently applied technique to detect and visualize various molecules in biological samples. Many protocols can be found in the literature and the websites of commercial antibody producers. This can result in a time-consuming and costly methodical work to establish "simple" antibody staining. We here summarize in a stepwise manner an easy-to-follow immunofluorescence staining protocol with an improved specific fluorescent signal and a reduced background and non-specific binding signal. This will help scientists to save time, effort, and antibody costs during the application of such a valuable technique. |
راعي المشروع | We thank Jutta Schüler, Susanne Kosanke, and Magdalena John for technical assistance as well as Pierre Gressens and his lab members for an initial protocol that served as a basis. We acknowledge support from the German Research Foundation (DFG, SFB665, SFB1315), the Open Access Publication Fund of Charité—Universitätsmedizin Berlin, and the German Academic Exchange Service (DAAD). |
اللغة | en |
الناشر | Frontiers Media |
الموضوع | antibodies imaging immunofluorescence microscopy paraffin sections |
النوع | Article |
رقم المجلد | 14 |
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