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AuthorZaqout, Sami
AuthorBecker, Lena-Luise
AuthorKaindl, Angela M
Available date2020-11-30T06:51:50Z
Publication Date2020
Publication NameFrontiers in Neuroanatomy
Identifierhttp://dx.doi.org/10.3389/fnana.2020.582218
CitationZaqout S, Becker LL, Kaindl AM. Immunofluorescence Staining of Paraffin Sections Step by Step. Front Neuroanat. 2020;14:582218. Published 2020 Nov 9. doi:10.3389/fnana.2020.582218
ISSN1662-5129
URIhttp://hdl.handle.net/10576/17124
AbstractImmunofluorescence staining is the most frequently applied technique to detect and visualize various molecules in biological samples. Many protocols can be found in the literature and the websites of commercial antibody producers. This can result in a time-consuming and costly methodical work to establish "simple" antibody staining. We here summarize in a stepwise manner an easy-to-follow immunofluorescence staining protocol with an improved specific fluorescent signal and a reduced background and non-specific binding signal. This will help scientists to save time, effort, and antibody costs during the application of such a valuable technique.
SponsorWe thank Jutta Schüler, Susanne Kosanke, and Magdalena John for technical assistance as well as Pierre Gressens and his lab members for an initial protocol that served as a basis. We acknowledge support from the German Research Foundation (DFG, SFB665, SFB1315), the Open Access Publication Fund of Charité—Universitätsmedizin Berlin, and the German Academic Exchange Service (DAAD).
Languageen
PublisherFrontiers Media
Subjectantibodies
imaging
immunofluorescence
microscopy
paraffin sections
TitleImmunofluorescence Staining of Paraffin Sections Step by Step.
TypeArticle
Volume Number14
dc.accessType Open Access


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