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المؤلفPerez-Lopez, Andres
المؤلفSundararaju, Sathyavathi
المؤلفAl-Mana, Hassan
المؤلفTsui, Kin Ming
المؤلفHasan, Mohammad Rubayet
المؤلفSuleiman, Mohammed
المؤلفJanahi, Mohammed
المؤلفAl Maslamani, Eman
المؤلفTang, Patrick
تاريخ الإتاحة2021-06-20T06:50:58Z
تاريخ النشر2020
اسم المنشورFrontiers in Microbiology
المعرّفhttp://dx.doi.org/10.3389/fmicb.2020.581711
الاقتباسPerez-Lopez A, Sundararaju S, Al-Mana H, Tsui KM, Hasan MR, Suleiman M, Janahi M, Al Maslamani E and Tang P (2020) Molecular Characterization of Extended-Spectrum β-Lactamase–Producing Escherichia coli and Klebsiella pneumoniae Among the Pediatric Population in Qatar. Front. Microbiol. 11:581711. doi: 10.3389/fmicb.2020.581711
الرقم المعياري الدولي للكتاب1664-302X
المعرّف581711
معرّف المصادر الموحدhttp://hdl.handle.net/10576/20747
الملخصAlthough extended-spectrum β-lactamase (ESBL)-producing Enterobacterales are a public health problem in the Arabian Peninsula, data on the molecular characteristic of their antimicrobial resistance determinants in children is limited. To determine the molecular characteristics of ESBL-producing and in the pediatric population of Qatar. Whole-genome sequencing was performed on ESBL-producing and isolates recovered from screening and clinical specimens from pediatric patients at Sidra Medicine in Doha from January to December 2018. A total of 327 ESBL producers were sequenced: 254 and 73 . Non-susceptibility rates to non-β-lactam antibiotics for both species were 18.1 and 30.1% for gentamicin, 0.8 and 4.1% for amikacin, 41.3 and 41.1% for ciprofloxacin, and 65.8 and 76.1% for cotrimoxazole. The most common sequence types (STs) were ST131 (16.9%), ST38 and ST10 (8.2% each) in and ST307 (9.7%), and ST45 and ST268 (6.9% each) in . CTX-M type ESBLs were found in all but one isolate, with CTX-M-15 accounting for 87.8%. Among other β-lactamases, TEM-1B and OXA-1 were coproduced in 41 and 19.6% of isolates. The most common plasmid-mediated quinolone resistance genes cocarried were (45.3%). Ninety percent of gentamicin non-susceptible isolates harbored genes encoding AAC(3) enzymes, mainly . Only two of 57 isolates harboring were non-susceptible to amikacin. Chromosomal mutations in genes encoding DNA gyrase and topoisomerase IV enzymes were detected in 96.2% fluoroquinolone-non-susceptible and 26.7% fluoroquinolone-non-susceptible . Our data show that CTX-M enzymes are largely the most prevalent ESBLs in children in Qatar with a predominance of CTX-M-15. Carbapenem-sparing options to treat ESBL infections are limited, given the frequent coproduction of OXA-1 and TEM-1B enzymes and coresistance to antibiotic classes other than β-lactams.
اللغةen
الناشرFrontiers Media
الموضوعCTX-M-15
E. coli
K. pneumoniae
children
extended-spectrum β-lactamases
fluoroquinolones
whole-genome sequencing
العنوانMolecular Characterization of Extended-Spectrum β-Lactamase-Producing and Among the Pediatric Population in Qatar
النوعArticle
رقم المجلد11
dc.accessType Open Access


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