Show simple item record

AuthorAl Mana, Hassan
AuthorJohar, Alreem A.
AuthorKassem, Issmat I.
AuthorEltai, Nahla O.
Available date2022-06-12T04:41:03Z
Publication Date2022-06
Publication NameAntibiotics
Identifierhttp://dx.doi.org/10.3390/antibiotics11060774
CitationAl Mana, H.; Johar, A.A.; Kassem, I.I.; Eltai, N.O. Transmissibility and Persistence of the Plasmid-Borne Mobile Colistin Resistance Gene, mcr-1, Harbored in Poultry-Associated E. coli. Antibiotics 2022, 11, 774. https://doi.org/10.3390/antibiotics11060774
URIhttp://hdl.handle.net/10576/32060
AbstractColistin, a last-resort antibiotic, is used to treat infections caused by multi-drug-resistant Gram-negative bacteria. Colistin resistance can emerge by acquiring the mobile colistin gene, mcr-1, usually plasmid borne. Studies on mcr-1 and its transmissibility are limited in the Middle East and North Africa (MENA) region. Here, we investigated the occurrence of mcr-1 in 18 previously collected Escherichia coli isolates collected from chicken samples in Qatar; whole-genome sequencing was performed to determine the location (plasmid-borne and chromosomal) of mcr-1 in the isolates. Additionally, we assessed the transmissibility of plasmid-borne mcr-1 and its cost on fitness in E. coli biofilms. Our results showed that the E. coli isolates belonged to different sequence types, indicating that mcr-1 was occurring in strains with diverse genetic backgrounds. In silico analysis and transformation assays showed that all the isolates carried mcr-1 on plasmids that were mainly IncI2 types. All the mcr-1 plasmids were found to be transmissible by conjugation. In biofilms, a significant reduction in the number of CFU (≈0.055 logs CFU/mL) and colistin resistance (≈2.19 log CFU/mL) was observed; however, the reduction in resistance was significantly larger, indicating that the plasmids incur a high fitness cost. To our knowledge, this is the first study that investigates mcr-1 transmissibility and persistence in Qatar. Our findings highlight that mcr has the potential to spread colistin resistance to potentially disparate strains and niches in Qatar, posing a risk that requires intervention.
SponsorThis research was funded by the Biomedical Research Centre, Qatar University, grant number (BRC-2022-ID-01) to Nahla O. Eltai.
Languageen
PublisherMDPI
Subjectcolistin
mcr-1
coli
fitness
transmission
biofilm
TitleTransmissibility and Persistence of the Plasmid-Borne Mobile Colistin Resistance Gene, mcr-1, Harbored in Poultry-Associated E. coli
TypeArticle
Issue Number6
Volume Number11
ESSN2079-6382
dc.accessType Open Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record