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AuthorKarin-Kujundzic, Valentina
AuthorCovarrubias-Pinto, Adriana
AuthorSkrtic, Anita
AuthorVranic, Semir
AuthorSerman, Ljiljana
Available date2022-10-09T10:51:23Z
Publication Date2022-12-01
Publication NameInternational Journal of Oncology
Identifierhttp://dx.doi.org/10.3892/ijo.2022.5435
CitationKarin‑Kujundzic V, Covarrubias‑Pinto A, Skrtic A, Vranic S and Serman L: New insight into the role of PTCH1 protein in serous ovarian carcinomas. Int J Oncol 61: 145, 2022
ISSN1019-6439
URIhttp://hdl.handle.net/10576/34957
AbstractThe Hedgehog (Hh) signaling pathway is essential for normal embryonic development, while its hyperactivation in the adult organism is associated with the development of various cancers. The role of the Hh signaling pathway in ovarian cancer has not been sufficiently investigated. Therefore, the present study investigated the role of protein patched homolog 1 (PTCH1), a component of the Hh signaling pathway, and changes in the promoter methylation status of the corresponding gene in a cohort of low‑(LGSC) and high‑grade serous ovarian carcinomas (HGSC) and HGSC cell lines (OVCAR8 and OVSAHO). PTCH1 protein expression level was analyzed using immunohistochemistry in tissue samples and immunofluorescence and western blotting in cell lines. DNA methylation patterns of the gene were analyzed using methylation‑specific PCR. PTCH1 protein expression was significantly higher in HGSCs and LGSCs compared with controls (healthy ovaries and fallopian tubes). Similarly, ovarian cancer cell lines exhibited significantly higher PTCH1 protein expression compared with a normal fallopian tube non‑ciliated epithelial cell line (FNE1). PTCH1 protein fragments of different molecular weights were detected in all cell lines, indicating possible proteolytic cleavage of this protein, resulting in the generation of soluble N‑terminal fragments that are translocated to the nucleus. DNA methylation of the gene promoter was exclusively detected in a proportion of HGSC (13.5%) but did not correlate with protein expression. PTCH1 protein was highly expressed in serous ovarian carcinoma tissues and cell lines, while promoter methylation was only detected in HGSC. Further investigation is required to elucidate the possible mechanisms of PTCH1 activation in serous ovarian carcinomas.
SponsorThis research was co‑financed by the European Union through the Europe Regional Development Fund, Operational Programme Competitiveness and Cohesion (grant no. KK.01.1.1.01.0008; Reproductive and Regenerative Medicine‑Exploring New Platforms and Potentials).
Languageen
PublisherSpandidos Publications
SubjectHedgehog signaling pathway
ovarian cancer
ovarian cancer cell lines
protein patched homolog 1
serous ovarian carcinoma
TitleNew insight into the role of PTCH1 protein in serous ovarian carcinomas.
TypeArticle
Pagination145-155
Issue Number6
Volume Number61
ESSN1791-2423
dc.accessType Open Access


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