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المؤلفStarykovych, Marina
المؤلفAntonyuk, Volodymyr
المؤلفNehrych, Tetyana
المؤلفNegrych, Nazar
المؤلفHorák, Daniel
المؤلفSouchelnytskyi, Serhiy
المؤلفKit, Oleg
المؤلفStoika, Rostyslav
المؤلفKit, Yuriy
تاريخ الإتاحة2023-03-27T07:23:01Z
تاريخ النشر2021-04-01
اسم المنشورBiomedical Chromatography
المعرّفhttp://dx.doi.org/10.1002/bmc.5029
الاقتباسStarykovych, M, Antonyuk, V, Nehrych, T, et al. Isolation and identification in human blood serum of the proteins possessing the ability to bind with 48 kDa form of unconventional myosin 1c and their possible diagnostic and prognostic value. Biomedical Chromatography. 2021; 35:e5029. https://doi.org/10.1002/bmc.5029
الرقم المعياري الدولي للكتاب02693879
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85096994228&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/41327
الملخصWe firstly identified 48 kDa molecular form of the unconventional myosin 1c (p48/Myo1C), and isolated it from blood serum of multiple sclerosis patients. The amount of p48/Myo1C in human blood serum correlated with some autoimmune, hemato-oncological and neurodegenerative diseases and thus may serve as a potential molecular biomarker. The biological functions of this protein in human blood remain unknown. Previously, we used the monodisperse magnetic poly (glycidyl methacrylate)(mag-PGMA–NH2) microspheres with immobilized 48/Myo1C and western-blot analysis, which allowed us to identify IgM and IgG immunoglobulins presenting an affinity to this protein. Here, we used mass spectrometry followed by the western blotting in order to identify other blood serum proteins with affinity to 48/Myo1C. The obtained data demonstrate that 48/Myo1C binds to component 3 of the complement and the antithrombin-III proteins. A combination of magnetic microparticle-based affinity chromatography with MALDI–TOF mass spectrometry and an in silico analysis provided an opportunity to identify the partners of interaction of 48/Myo1C with other proteins, in particular those participating in complement and coagulation cascades.
اللغةen
الناشرWiley
الموضوع48/Myo1C protein
affinity isolation
human blood serum
magnetic microparticles
mass spectrometry
western blotting
العنوانIsolation and identification in human blood serum of the proteins possessing the ability to bind with 48 kDa form of unconventional myosin 1c and their possible diagnostic and prognostic value
النوعArticle
رقم العدد4
رقم المجلد35
ESSN1099-0801
dc.accessType Abstract Only


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