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AuthorVijayanand, Sharon
AuthorPatil, Smital
AuthorMenon, Ipshita
AuthorBraz Gomes, Keegan
AuthorKale, Akanksha
AuthorBagwe, Priyal
AuthorUddin, Mohammad N.
AuthorZughaier, Susu M.
AuthorD’Souza, Martin J.
Available date2023-05-03T09:27:46Z
Publication Date2023-03-09
Publication NamePharmaceutics
Identifierhttp://dx.doi.org/10.3390/pharmaceutics15030895
CitationVijayanand, S., Patil, S., Menon, I., Braz Gomes, K., Kale, A., Bagwe, P., ... & D’Souza, M. J. (2023). An Adjuvanted Inactivated SARS-CoV-2 Microparticulate Vaccine Delivered Using Microneedles Induces a Robust Immune Response in Vaccinated Mice. Pharmaceutics, 15(3), 895.
ISSN1999-4923
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85151379093&origin=inward
URIhttp://hdl.handle.net/10576/42273
AbstractSARS-CoV-2, the causal agent of COVID-19, is a contagious respiratory virus that frequently mutates, giving rise to variant strains and leading to reduced vaccine efficacy against the variants. Frequent vaccination against the emerging variants may be necessary; thus, an efficient vaccination system is needed. A microneedle (MN) vaccine delivery system is non-invasive, patient-friendly, and can be self-administered. Here, we tested the immune response produced by an adjuvanted inactivated SARS-CoV-2 microparticulate vaccine administered via the transdermal route using a dissolving MN. The inactivated SARS-CoV-2 vaccine antigen and adjuvants (Alhydrogel® and AddaVax™) were encapsulated in poly(lactic-co-glycolic acid) (PLGA) polymer matrices. The resulting MP were approximately 910 nm in size, with a high percentage yield and percent encapsulation efficiency of 90.4%. In vitro, the vaccine MP was non-cytotoxic and increased the immunostimulatory activity measured as nitric oxide release from dendritic cells. The adjuvant MP potentiated the immune response of the vaccine MP in vitro. In vivo, the adjuvanted SARS-CoV-2 MP vaccine induced high levels of IgM, IgG, IgA, IgG1, and IgG2a antibodies and CD4+ and CD8+ T-cell responses in immunized mice. In conclusion, the adjuvanted inactivated SARS-CoV-2 MP vaccine delivered using MN induced a robust immune response in vaccinated mice.
Languageen
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
Subjectantibody response
cytotoxicity
immunogenicity
microneedles
microparticles
SARS-CoV-2
T-cell response
TitleAn Adjuvanted Inactivated SARS-CoV-2 Microparticulate Vaccine Delivered Using Microneedles Induces a Robust Immune Response in Vaccinated Mice
TypeArticle
Issue Number3
Volume Number15
ESSN1999-4923
dc.accessType Open Access


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