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المؤلفJami, Sina
المؤلفDeuis, Jennifer R.
المؤلفKlasfauseweh, Tabea
المؤلفCheng, Xiaoyang
المؤلفKurdyukov, Sergey
المؤلفChung, Felicity
المؤلفOkorokov, Andrei L.
المؤلفLi, Shengnan
المؤلفZhang, Jiangtao
المؤلفCristofori-Armstrong, Ben
المؤلفIsrael, Mathilde R.
المؤلفJu, Robert J.
المؤلفRobinson, Samuel D.
المؤلفZhao, Peng
المؤلفRagnarsson, Lotten
المؤلفAndersson, Åsa
المؤلفTran, Poanna
المؤلفSchendel, Vanessa
المؤلفMcMahon, Kirsten L.
المؤلفTran, Hue N. T.
المؤلفChin, Yanni K.-Y.
المؤلفZhu, Yifei
المؤلفLiu, Junyu
المؤلفCrawford, Theo
المؤلفPurushothamvasan, Saipriyaa
المؤلفHabib, Abdella M.
المؤلفAndersson, David A.
المؤلفRash, Lachlan D.
المؤلفWood, John N.
المؤلفZhao, Jing
المؤلفStehbens, Samantha J.
المؤلفMobli, Mehdi
المؤلفLeffler, Andreas
المؤلفJiang, Daohua
المؤلفCox, James J.
المؤلفWaxman, Stephen G.
المؤلفDib-Hajj, Sulayman D.
المؤلفGregory Neely, G.
المؤلفDurek, Thomas
المؤلفVetter, Irina
تاريخ الإتاحة2023-05-15T06:13:54Z
تاريخ النشر2023
اسم المنشورNature Communications
المصدرScopus
الرقم المعياري الدولي للكتاب2041-1723
معرّف المصادر الموحدhttp://dx.doi.org/10.1038/s41467-023-37963-2
معرّف المصادر الموحدhttp://hdl.handle.net/10576/42691
الملخصVoltage-gated sodium (NaV) channels are critical regulators of neuronal excitability and are targeted by many toxins that directly interact with the pore-forming α subunit, typically via extracellular loops of the voltage-sensing domains, or residues forming part of the pore domain. Excelsatoxin A (ExTxA), a pain-causing knottin peptide from the Australian stinging tree Dendrocnide excelsa, is the first reported plant-derived NaV channel modulating peptide toxin. Here we show that TMEM233, a member of the dispanin family of transmembrane proteins expressed in sensory neurons, is essential for pharmacological activity of ExTxA at NaV channels, and that co-expression of TMEM233 modulates the gating properties of NaV1.7. These findings identify TMEM233 as a previously unknown NaV1.7-interacting protein, position TMEM233 and the dispanins as accessory proteins that are indispensable for toxin-mediated effects on NaV channel gating, and provide important insights into the function of NaV channels in sensory neurons.
راعي المشروعThis work was supported by the University of Queensland Protein Production Facility and the Australian Cancer Research Foundation (ACRF) Cancer Biology Imaging Facility. Financial support was provided by the Australian Research Council (ARC; DP200102377, CE200100012) and the Australian National Health and Medical Research Council (NHMRC) fellowship to I.V. (APP1162503), Project grant (APP1162597) to M.M. and L.D.R., and Medical Research Council grant to J.J.C. and A.L.O. (MR/R011737/1). B.C.A. received funding from an NHMRC CJ Martin Fellowship (APP1162427). J.R.D. received funding through an ARC DECRA fellowship (DE210100422). S.D.H. and S.G.W. were supported by Merit Review Award B9253-C from the U.S. Dept. of Veterans Affairs Rehabilitation Service; The Center for Neuroscience & Regeneration Research is a Collaboration of the Paralyzed Veterans of America with Yale University. M.R.I. and D.A.A. received grants from the UK Medical Research Council (MR/S003428/1, MR/W002426/1). J.J.C., S.L., A.M.H., J.Zhao. and J.N.W. were supported by the Wellcome Trust (200183). A.M.H. receives funding from Qatar University (QUSD-CMED-2018/9-3; QUCG-CMED-19/20-4). We thank Dr. Patrick Walsh and Vincent Truong from Anatomic Inc. for helpful discussions on the gene expression profile of Anatomic Inc. iPSC-derived sensory neurons; Virginia Nink (Queensland Brain Institute, University of Queensland) for expert assistance with flow cytometry; and Prof Jenny Stow and Dr Lin Luo (Institute for Molecular Bioscience, University of Queensland) for provision of FANTOM Riken cDNA plasmids.
اللغةen
الناشرNature Research
الموضوعIon channels in the nervous system
Membrane proteins
Permeation and transport
Plant sciences
Sodium channels
العنوانPain-causing stinging nettle toxins target TMEM233 to modulate NaV1.7 function
النوعArticle
رقم العدد1
رقم المجلد14
dc.accessType Open Access


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