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AuthorKannan, Surya
AuthorSouchelnytskyi, Serhiy
Available date2023-07-12T09:59:52Z
Publication Date2022-12-12
Publication NameElectronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (EJIFCC)
Identifierhttp://dx.doi.org/110.3389/fimmu.2022.882718
CitationKannan, S., & Souchelnytskyi, S. (2022). The exposure to human breast cancer cells altered 14 post-translational modifications of human serum albumin. EJIFCC, 33(4), 295.
ISSN1650-3414
URIhttp://hdl.handle.net/10576/45450
AbstractSerum albumin is in contact with practically all cells in the human body, including tumor cells in cancer patients. The purpose of this study was to explore whether cancer cells affect post-translational modifications (PTMs) of albumin. Mass spectrometry was used to identify the PTMs. Purified human serum albumin was incubated with human breast cancer cells MDA-MB-231, MDA-MB-468, MCF7, or kept in water or in cell culture media. PTMs which were affected upon exposure of the albumin to cancer cells were identified. Three-dimensional analysis was performed to locate PTMs in albumin. We report here that an exposure to human breast cancer cells affected post-translational modifications (PTMs) of 14 peptides of human serum albumin (HSA). PTMs at 8 peptides were observed upon exposure of HSA to metastatic MDA-MB-231 and MDA-MB-468 breast cancer cells. PTMs at another 6 peptides were lost in MDA-MB-231 and MDA-MB-468 cells, while these 6 PTMs were observed in HSA exposed to conditionally tumorigenic MCF7 cells, or in HSA kept in water or a cell culture medium. Cancer cell altered phosphorylation, deamidation followed by methylation, acetylation, myristylation, palmitoylation, methylation, cysteine persulfide, and S-6-FMN cysteine modifications were detected in HSA. These PTMs locate predominantly in IB and IIA domains of HSA. Three-dimensional analysis showed that this region corresponds to the lipid-binding site and Sudlow's site 1. Data reported here show that 14 PTMs of human serum albumin can be modified upon its exposure to human breast cancer cells.
SponsorPartial support by the “Ove’s Minnesfond”, QUCG-CMED-20/21-1, QUCG-CMED-18/19-2, and QUST-2-CMED-2020-12 to S.S. is acknowledged.
Languageen
PublisherInternational Federation of Clinical Chemistry and Laboratory Medicine
Subjecthuman serum albumin
post-translational modifications
TitleThe Exposure to Human Breast Cancer Cells Altered 14 Post-Translational Modifications of Human Serum Albumin.
TypeArticle
Pagination295-308
Issue Number4
Volume Number33
dc.accessType Open Access


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