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المؤلفSalloum-Asfar, Salam
المؤلفEngelke, Rudolf
المؤلفMousa, Hanaa
المؤلفGoswami, Neha
المؤلفThompson, I. Richard
المؤلفPalangi, Freshteh
المؤلفKamal, Kamal
المؤلفAl-Noubi, Muna N.
المؤلفSchmidt, Frank
المؤلفAbdulla, Sara A.
المؤلفEmara, Mohamed M.
تاريخ الإتاحة2023-07-30T11:41:36Z
تاريخ النشر2021-10-15
اسم المنشورStem Cells International
المعرّفhttp://dx.doi.org/10.1155/2021/8274936
الاقتباسSalloum-Asfar, S., Engelke, R., Mousa, H., Goswami, N., Thompson, I. R., Palangi, F., ... & Emara, M. M. (2021). Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells. Stem Cells International, 2021.
الرقم المعياري الدولي للكتاب1687-966X
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85118280508&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/46405
الملخصStress granules (SGs) are assemblies of selective messenger RNAs (mRNAs), translation factors, and RNA-binding proteins in small untranslated messenger ribonucleoprotein (mRNP) complexes in the cytoplasm. Evidence indicates that different types of cells have shown different mechanisms to respond to stress and the formation of SGs. In the present work, we investigated how human-induced pluripotent stem cells (hiPSCs/IMR90-1) overcome hyperosmotic stress compared to a cell line that does not harbor pluripotent characteristics (SH-SY5Y cell line). Gradient concentrations of NaCl showed a different pattern of SG formation between hiPSCs/IMR90-1 and the nonpluripotent cell line SH-SY5Y. Other pluripotent stem cell lines (hiPSCs/CRTD5 and hESCs/H9 (human embryonic stem cell line)) as well as nonpluripotent cell lines (BHK-21 and MCF-7) were used to confirm this phenomenon. Moreover, the formation of hyperosmotic SGs in hiPSCs/IMR90-1 was independent of eIF2α phosphorylation and was associated with low apoptosis levels. In addition, a comprehensive proteomics analysis was performed to identify proteins involved in regulating this specific pattern of hyperosmotic SG formation in hiPSCs/IMR90-1. We found possible implications of microtubule organization on the response to hyperosmotic stress in hiPSCs/IMR90-1. We have also unveiled a reduced expression of tubulin that may protect cells against hyperosmolarity stress while inhibiting SG formation without affecting stem cell self-renewal and pluripotency. Our observations may provide a possible cellular mechanism to better understand SG dynamics in pluripotent stem cells.
اللغةen
الناشرHindawi
الموضوعIMR-90 cell line
selective messenger RNA
العنوانHyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells
النوعArticle
رقم المجلد2021
ESSN1687-9678
dc.accessType Open Access


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