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AuthorSalloum-Asfar, Salam
AuthorEngelke, Rudolf
AuthorMousa, Hanaa
AuthorGoswami, Neha
AuthorThompson, I. Richard
AuthorPalangi, Freshteh
AuthorKamal, Kamal
AuthorAl-Noubi, Muna N.
AuthorSchmidt, Frank
AuthorAbdulla, Sara A.
AuthorEmara, Mohamed M.
Available date2023-07-30T11:41:36Z
Publication Date2021-10-15
Publication NameStem Cells International
Identifierhttp://dx.doi.org/10.1155/2021/8274936
CitationSalloum-Asfar, S., Engelke, R., Mousa, H., Goswami, N., Thompson, I. R., Palangi, F., ... & Emara, M. M. (2021). Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells. Stem Cells International, 2021.
ISSN1687-966X
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85118280508&origin=inward
URIhttp://hdl.handle.net/10576/46405
AbstractStress granules (SGs) are assemblies of selective messenger RNAs (mRNAs), translation factors, and RNA-binding proteins in small untranslated messenger ribonucleoprotein (mRNP) complexes in the cytoplasm. Evidence indicates that different types of cells have shown different mechanisms to respond to stress and the formation of SGs. In the present work, we investigated how human-induced pluripotent stem cells (hiPSCs/IMR90-1) overcome hyperosmotic stress compared to a cell line that does not harbor pluripotent characteristics (SH-SY5Y cell line). Gradient concentrations of NaCl showed a different pattern of SG formation between hiPSCs/IMR90-1 and the nonpluripotent cell line SH-SY5Y. Other pluripotent stem cell lines (hiPSCs/CRTD5 and hESCs/H9 (human embryonic stem cell line)) as well as nonpluripotent cell lines (BHK-21 and MCF-7) were used to confirm this phenomenon. Moreover, the formation of hyperosmotic SGs in hiPSCs/IMR90-1 was independent of eIF2α phosphorylation and was associated with low apoptosis levels. In addition, a comprehensive proteomics analysis was performed to identify proteins involved in regulating this specific pattern of hyperosmotic SG formation in hiPSCs/IMR90-1. We found possible implications of microtubule organization on the response to hyperosmotic stress in hiPSCs/IMR90-1. We have also unveiled a reduced expression of tubulin that may protect cells against hyperosmolarity stress while inhibiting SG formation without affecting stem cell self-renewal and pluripotency. Our observations may provide a possible cellular mechanism to better understand SG dynamics in pluripotent stem cells.
Languageen
PublisherHindawi
SubjectIMR-90 cell line
selective messenger RNA
TitleHyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells
TypeArticle
Volume Number2021
ESSN1687-9678
dc.accessType Open Access


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