Host specificity of pseudomonas aeruginosa isolates from patients with cystic fibrosis and patients from different clinical backgrounds.
Author | AbdulWahab, Atqah |
Author | Taj-Aldeen, Saad J |
Author | Ibrahim, Emad |
Author | Hussain, Shaikha |
Author | Muhammed, Ramees |
Author | Ahmed, Irshad |
Author | Abu-Madi, Marawan |
Available date | 2017-12-31T08:53:57Z |
Publication Date | 2014 |
Publication Name | European Respiratory Journal |
Citation | Wahab, A. A., Taj-Aldeen, S. J., Ibrahim, E., Hussain, S., Mohammed, R., Ahmed, I., & Abu-Madi, M. (2014). Host specificity of pseudomonas aeruginosa isolates from patients with cystic fibrosis and patients from different clinical backgrounds. European Respiratory Journal, 44(Suppl 58), P1211. |
ISSN | 0903-1936 |
Abstract | Background: Pseudomonas aeruginosa is one of the primary pathogens being isolated more frequently in cystic fibrosis (CF) and exhibits innate resistant to a wide range of antibiotics. Purpose: To determine whether the highly prevalent genotypes of P. aeruginosa are specifically linked to CF patients, isolates from non-CF patients and environment sources were genotypically analyzed. Methods: Collections of P. aeruginosa from lower respiratory secretions (n= 57) were genotyped using pulse-field gel electrophoresis (PFGE). Phenotypic screening for antibiotic susceptibility was performed for the common antimicrobial agents by Etest and automated Phoenix. Results: P. aeruginosa isolates from CF (n=39), non-CF patients (n=13), and environment sources (n=5) were analyzed. The population structure of P. aeruginosa is highly diverse and population specific. All the strains fall among 3 major clusters. Cluster A contained 16 isolates from CF patients and 2 environmental; cluster B contained 12 isolates from CF and one environmental while cluster C contained all the isolates from non CF patients and one environmental. The majority of P. aeruginosa strains in CF were resistant to ciprofloxacin (25.7%) followed by amikacin and gentamicin (each 23.6%). Whereas, the majority of isolates from non-CF were resistant to meropenem (69%), which grouped in cluster C. Conclusion: The fingerprints obtained with P. aeruginosa isolates from CF patients have a high degree of similarity, suggesting specific adaptation of these two clones to CF lung. The third non-CF cluster has different clonal origin, indicating specific clustering in both location and patient group. |
Language | en |
Publisher | European Respiratory Society: ERJ |
Subject | cystic fibrosis Bacteria Genetics |
Type | Article |
Pagination | 1211 |
ESSN | 1399-3003 |
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