Bordetella pertussis risA, but not risS, is required for maximal expression of Bvg-repressed genes

QSpace/Manakin Repository

Bordetella pertussis risA, but not risS, is required for maximal expression of Bvg-repressed genes

Show full item record


Title: Bordetella pertussis risA, but not risS, is required for maximal expression of Bvg-repressed genes
Author: Stenson, T.H.; Allen, A.G.; Al-Meer, J.A.; Maskell, D.; Peppler, M.S.
Abstract: Expression of virulence determinants by Bordetella pertussis, the primary etiological agent of whooping cough, is regulated by the BvgAS two-component regulatory system. The role of a second two-component regulatory system, encoded by risAS, in this process is not defined. Here, we show that mutation of B. pertussis risA does not affect Bvg-activated genes or proteins. However, mutation of risA resulted in greatly diminished expression of Bvg-repressed antigens and decreased transcription of Bvg-repressed genes. In contrast, mutation of risS had no effect on the expression of Bvg-regulated molecules. Mutation of risA also resulted in decreased bacterial invasion in a HeLa cell model. However, decreased invasion could not be attributed to the decreased expression of Bvg-repressed products, suggesting that mutation of risA may affect the expression of a variety of genes. Unlike the risAS operons in B. parapertussis and B. bronchiseptica, B. pertussis risS is a pseudogene that encodes a truncated RisS sensor. Deletion of the intact part of the B. pertussis risS gene does not affect the expression of risA-dependent, Bvg-repressed genes. These observations suggest that RisA activation occurs through cross-regulation by a heterologous system.
URI: http://dx.doi.org/10.1128/IAI.73.9.5995-6004.2005
http://hdl.handle.net/10576/10538
Date: 2005-09

Files in this item

Files Size Format View Description
5995.pdf 192.8Kb PDF View/Open Main article

This item appears in the following Collection(s)

Show full item record

Search QSpace


Advanced Search

Browse

My Account