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المؤلفAl-Awar A.
المؤلفAlm?si N.
المؤلفSzab? R.
المؤلفTakacs I.
المؤلفMurlasits Z.
المؤلفSz?cs G.
المؤلفT?r?k S.
المؤلفP?sa A.
المؤلفVarga C.
المؤلفKupai K.
تاريخ الإتاحة2020-02-24T08:57:14Z
تاريخ النشر2018
اسم المنشورInternational Journal of Molecular Sciences
المصدرScopus
الرقم المعياري الدولي للكتاب16616596
معرّف المصادر الموحدhttp://dx.doi.org/10.3390/ijms19103226
معرّف المصادر الموحدhttp://hdl.handle.net/10576/13012
الملخصDipeptidyl peptidase-4 (DPP-4) inhibitors are a class of oral anti-diabetic drugs, implicated in pleiotropic secondary cardioprotective effects. The aim of the study was to unveil the unknown and possible cardioprotective targets that can be exerted by sitagliptin (Sitg) against ischemia-reperfusion (I/R) injury. Male wistar rats received 2 weeks? Sitg oral treatment of different doses (25, 50, 100, and 150 mg/kg/day), or saline as a Control. Hearts were then isolated and subjected to two different I/R injury protocols: 10 min perfusion, 45 min regional ischemia, and 120 min reperfusion for infarct size (IS) measurement, or: 10 min perfusion, 45 min regional ischemia and 10 min reperfusion for biochemical analysis: nitric oxide synthases (NOSs) and DPP-4 activity, glucagon-like peptide-1 (GLP-1), Calcium, transient receptor potential vanilloid (TRPV)-1 and calcitonin gene-related peptide (CGRP) levels, transient receptor potential canonical (TRPC)-1 and e-NOS protein expression. NOS inhibitor (L-NAME) and TRPV-1 inhibitor (Capsazepine) were utilized to confirm the implication of both signaling mechanisms in DPP-4 inhibition-induced at the level of IS. Findings show that Sitg (50 mg) resulted in significant decrease in IS and DPP-4 activity, and significant increase in GLP-1, NOS activity, e-NOS expression, TRPV-1 level and TRPC-1 expression, compared to controls. Results of CGRP are in line with TRPV-1, as a downstream regulatory effect. NOS system and transient receptor potential (TRP) channels can contribute to DPP-4 inhibition-mediated cardioprotection against I/R injury using Sitagliptin.
راعي المشروعThis research was funded by GINOP grant number 2.3.2-15-2016-00035.
اللغةen
الناشرMDPI AG
الموضوعCalcitonin gene related peptide
Dipeptidyl-peptidase-4
DPP-4 inhibitors
Endothelial nitric oxide synthase
Infarct size
Ischemia-reperfusion injury
NOS activity
Transient receptor potential channels
العنوانNovel potentials of the DPP-4 inhibitor sitagliptin against ischemia-reperfusion (I/R) injury in rat ex-vivo heart model
النوعArticle
رقم العدد10
رقم المجلد19
dc.accessType Abstract Only


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