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AuthorHadi M., Yassine
AuthorAl-Jighefee, Hadeel
AuthorAl-Sadeq, Duaa W.
AuthorDargham, Soha R.
AuthorYounes, Salma N.
AuthorShurrab, Farah
AuthorMarei, Reham M.
AuthorHssain, Ali Ait
AuthorTaleb, Sara
AuthorAlhussain, Hashim A.
AuthorAl-Nesf, Maryiam A.
AuthorAl-Khal, Abdullatif
AuthorQotba, Hamda
AuthorAlthani, Asmaa A.
AuthorTang, Patrick
AuthorAbu-Raddad, Laith J.
AuthorNasrallah, Gheyath K.
Available date2020-11-03T04:54:33Z
Publication Date2020-10-27
Publication NameInternational Journal of Infectious Diseasesen_US
Identifierhttp://dx.doi.org/10.1016/j.ijid.2020.10.042
CitationYassine HM, Al-Jighefee H, Al-Sadeq DW, Dargham SR, Younes SN, Shurrab F, Marei RM, Hssain AA, Taleb S, Alhussain HA, Al-Nesf MA, Al-Khal A, Qotba H, Althani AA, Tang P, Abu-Raddad LJ, Nasrallah GK. Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies. Int J Infect Dis. 2020 Oct 27:S1201-9712(20)32246-3. doi: 10.1016/j.ijid.2020.10.042. Epub ahead of print. PMID: 33127504; PMCID: PMC7590641.
ISSN12019712
URIhttps://www.sciencedirect.com/science/article/pii/S1201971220322463?v=s5
URIhttp://hdl.handle.net/10576/16855
AbstractObjectivesTo evaluate and compare the performances of five commercial ELISA assays (EDI, AnshLabs, Dia.Pro, NovaTec, and Lionex) for detecting anti-SARS-CoV-2 IgG. Methods70 negative control samples (collected before the COVID-19 pandemic) and samples from 101 RT-PCR-confirmed SARS-CoV-2 patients (collected at different time points from symptoms onset: ≤7, 8-14, and >14 days) were used to compare the sensitivity, specificity, agreement, positive and negative predictive values of each assay with RT-PCR. A concordance assessment between the five assays was also conducted. Cross-reactivity with other HCoV, non-HCoV respiratory viruses, non-respiratory viruses, and nuclear antigens was investigated. ResultsLionex showed the highest specificity (98.6%, 95%CI: 92.3-99.8), followed by EDI and Dia.Pro (97.1%, 95%CI: 90.2-99.2), NovaTec (85.7%, 95%CI: 75.7-92.1), then AnshLabs (75.7%, 95%CI: 64.5-84.2). All ELISA kits cross-reacted with one anti-MERS IgG positive sample except Lionex. The sensitivity was low during the early stages of the disease but improved over time. After 14 days from symptoms onset, Lionex and NovaTec showed the highest sensitivity at 87.9% (95%CI: 72.7-95.2) and 86.4% (95%CI: 78.5-91.7), respectively. The agreement with RT-PCR results based on Cohen’s kappa was as follows: Lionex (0.89)> NovaTec (0.70)> Dia.Pro (0.69)> AnshLabs (0.63)> EDI (0.55). ConclusionThe Lionex ELISA, which measures antibodies solely to the S1 protein, demonstrated the best performance.
SponsorThis work was made possible by grant No. RRC-2-032 from the Qatar National Research Fund (a member of Qatar Foundation). The statements made herein are solely the responsibility of the authors. GKN would like to acknowledge funds from Qatar University's internal grant QUERG-CMED-2020-2.
Languageen
PublisherElsevier
SubjectCOVID-19
SubjectSARS-CoV-2
SubjectSerology
SubjectIgG
SubjectELISA
SubjectSensitivity
SubjectSpecificity
TitlePerformance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies
TypeArticle
Open Access user License http://creativecommons.org/licenses/by-nc-nd/4.0/


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