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المؤلفHadi M., Yassine
المؤلفAl-Jighefee, Hadeel
المؤلفAl-Sadeq, Duaa W.
المؤلفDargham, Soha R.
المؤلفYounes, Salma N.
المؤلفShurrab, Farah
المؤلفMarei, Reham M.
المؤلفHssain, Ali Ait
المؤلفTaleb, Sara
المؤلفAlhussain, Hashim A.
المؤلفAl-Nesf, Maryiam A.
المؤلفAl-Khal, Abdullatif
المؤلفQotba, Hamda
المؤلفAlthani, Asmaa A.
المؤلفTang, Patrick
المؤلفAbu-Raddad, Laith J.
المؤلفNasrallah, Gheyath K.
تاريخ الإتاحة2020-11-03T04:54:33Z
تاريخ النشر2020-10-27
اسم المنشورInternational Journal of Infectious Diseases
المعرّفhttp://dx.doi.org/10.1016/j.ijid.2020.10.042
الاقتباسYassine HM, Al-Jighefee H, Al-Sadeq DW, Dargham SR, Younes SN, Shurrab F, Marei RM, Hssain AA, Taleb S, Alhussain HA, Al-Nesf MA, Al-Khal A, Qotba H, Althani AA, Tang P, Abu-Raddad LJ, Nasrallah GK. Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies. Int J Infect Dis. 2020 Oct 27:S1201-9712(20)32246-3. doi: 10.1016/j.ijid.2020.10.042. Epub ahead of print. PMID: 33127504; PMCID: PMC7590641.
الرقم المعياري الدولي للكتاب12019712
معرّف المصادر الموحدhttps://www.sciencedirect.com/science/article/pii/S1201971220322463?v=s5
معرّف المصادر الموحدhttp://hdl.handle.net/10576/16855
الملخصObjectivesTo evaluate and compare the performances of five commercial ELISA assays (EDI, AnshLabs, Dia.Pro, NovaTec, and Lionex) for detecting anti-SARS-CoV-2 IgG. Methods70 negative control samples (collected before the COVID-19 pandemic) and samples from 101 RT-PCR-confirmed SARS-CoV-2 patients (collected at different time points from symptoms onset: ≤7, 8-14, and >14 days) were used to compare the sensitivity, specificity, agreement, positive and negative predictive values of each assay with RT-PCR. A concordance assessment between the five assays was also conducted. Cross-reactivity with other HCoV, non-HCoV respiratory viruses, non-respiratory viruses, and nuclear antigens was investigated. ResultsLionex showed the highest specificity (98.6%, 95%CI: 92.3-99.8), followed by EDI and Dia.Pro (97.1%, 95%CI: 90.2-99.2), NovaTec (85.7%, 95%CI: 75.7-92.1), then AnshLabs (75.7%, 95%CI: 64.5-84.2). All ELISA kits cross-reacted with one anti-MERS IgG positive sample except Lionex. The sensitivity was low during the early stages of the disease but improved over time. After 14 days from symptoms onset, Lionex and NovaTec showed the highest sensitivity at 87.9% (95%CI: 72.7-95.2) and 86.4% (95%CI: 78.5-91.7), respectively. The agreement with RT-PCR results based on Cohen’s kappa was as follows: Lionex (0.89)> NovaTec (0.70)> Dia.Pro (0.69)> AnshLabs (0.63)> EDI (0.55). ConclusionThe Lionex ELISA, which measures antibodies solely to the S1 protein, demonstrated the best performance.
راعي المشروعThis work was made possible by grant No. RRC-2-032 from the Qatar National Research Fund (a member of Qatar Foundation). The statements made herein are solely the responsibility of the authors. GKN would like to acknowledge funds from Qatar University's internal grant QUERG-CMED-2020-2.
اللغةen
الناشرElsevier
الموضوعCOVID-19
SARS-CoV-2
Serology
IgG
ELISA
Sensitivity
Specificity
العنوانPerformance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies
النوعArticle
Open Access user License http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.accessType Open Access


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